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product-image-AAA78584_WB10.jpg WB (Western Blot) (Figure-4: Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in (1) MIapica-2, 0.1 ug/ml in (2) PANC-1, 0.01 ug/ml in (3) HCT-116 and (4) PC3 lysates.)

Mouse anti-Human CK19 Monoclonal Antibody | anti-CK19 antibody

Monoclonal Antibody to CK19 (Clone: A53-B/A2)

Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein G Chromatography
Synonyms
CK19, Antibody; Monoclonal Antibody to CK19 (Clone: A53-B/A2); KRT19; CK19; anti-CK19 antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a Kappa
Clone Number
A53-B/A2
Purity/Purification
Protein G Chromatography
Form/Format
Purified. 25ug in 50 ul/100ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-CK19 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Human breast cancer MCF-7 cells were used as the immunogen for the Keratin-19 antibody.
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months. For long-term storage; store at -20 degree C. Avoid repeated freeze and thaw cycles.

WB (Western Blot)

(Figure-4: Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in (1) MIapica-2, 0.1 ug/ml in (2) PANC-1, 0.01 ug/ml in (3) HCT-116 and (4) PC3 lysates.)

product-image-AAA78584_WB10.jpg WB (Western Blot) (Figure-4: Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in (1) MIapica-2, 0.1 ug/ml in (2) PANC-1, 0.01 ug/ml in (3) HCT-116 and (4) PC3 lysates.)

Application Data

(Figure-3: Immunohistochemical analysis of CK19 in human Pancrease, Duct using CK19 antibody (Clone: A53-B/A2) at 10 ug/ml.)

product-image-AAA78584_AD11.jpg Application Data (Figure-3: Immunohistochemical analysis of CK19 in human Pancrease, Duct using CK19 antibody (Clone: A53-B/A2) at 10 ug/ml.)

IHC (Immunohiostchemistry)

(Figure-2: Immunohistochemical analysis of CK19 in human colon cancer using CK19 antibody (Clone: A53-B/A2) at 1:100 dilution.)

product-image-AAA78584_IHC13.jpg IHC (Immunohiostchemistry) (Figure-2: Immunohistochemical analysis of CK19 in human colon cancer using CK19 antibody (Clone: A53-B/A2) at 1:100 dilution.)

WB (Western Blot)

(Figure-1: Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in 1) HepG2 and 2) MCF7 lysates.)

product-image-AAA78584_WB15.jpg WB (Western Blot) (Figure-1: Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in 1) HepG2 and 2) MCF7 lysates.)
Related Product Information for anti-CK19 antibody
Keratin-19 (K19) is a type I intermediate filament protein and one of the best characterized of the keratins expressed in mature striated muscle. Keratin-19 is expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium. Keratin-19 has been used as a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Immunohistochemical data has shown that Keratin-19 may be uses as a marker for human skin stem cells.
Product Categories/Family for anti-CK19 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,106 Da
NCBI Official Full Name
keratin, type I cytoskeletal 19
NCBI Official Synonym Full Names
keratin 19
NCBI Official Symbol
KRT19
NCBI Official Synonym Symbols
K19; CK19; K1CS
NCBI Protein Information
keratin, type I cytoskeletal 19; CK-19; keratin-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; 40-kDa keratin intermediate filament
UniProt Protein Name
Keratin, type I cytoskeletal 19
UniProt Gene Name
KRT19
UniProt Synonym Gene Names
CK-19; K19
UniProt Entry Name
K1C19_HUMAN

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Product Notes

The CK19 krt19 (Catalog #AAA78584) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CK19 (Clone: A53-B/A2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CK19 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CK19 krt19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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