Application Data
(BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-PE)
Rat anti-Mouse CD49d/VLA-4 Monoclonal Antibody
Rat Anti-Mouse CD49d/VLA-4a-BIOT
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD49d/VLA-4, Antibody; Rat Anti-Mouse CD49d/VLA-4a-BIOT; Rat Anti-Mouse CD49d/VLA-4a - Biotin; Rat Anti-Mouse CD49d/VLA-4; anti-CD49d/VLA-4 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
PS/2
Specificity
Mouse/Human CD49d; Mr 150 kDa
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for anti-CD49d/VLA-4 antibody
Blocking, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Immunogen
P815 cells
Preparation and Storage
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C.
Application Data
(BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-PE)
Application Data
(BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-PE)
Related Product Information for anti-CD49d/VLA-4 antibody
VLA-4 belongs to the β1-integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/Pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. Antibody PS/2 also reacts with human VLA-4.
References
1. Miyake, K., I. L. Weissman, J. S. Greenberger, and P. W. Kincade. 1991. J. Exp. Med. 173:599.
2. Christensen, J. P., E. C. Andersson, A. Scheynius, O. Marker, and A. R. Thomsen. 1995. J. Immunol. 154:5293.
3. Henseleit, U., K. Steinbrink, C. Sunderkotter, M. Goebeler, J. Roth, and C. Sorg. 1994. Exp. Dermatol. 3:249
4. Lauri, D., C. De Giovanni, T. Biondelli, E. Lalli, L. Landuzzi, A. Facchini, G. Nicoletti, P. Nanni, E. Dejana, and P. L. Lollini. 1993. Br. J. Cancer 68:862.
2. Christensen, J. P., E. C. Andersson, A. Scheynius, O. Marker, and A. R. Thomsen. 1995. J. Immunol. 154:5293.
3. Henseleit, U., K. Steinbrink, C. Sunderkotter, M. Goebeler, J. Roth, and C. Sorg. 1994. Exp. Dermatol. 3:249
4. Lauri, D., C. De Giovanni, T. Biondelli, E. Lalli, L. Landuzzi, A. Facchini, G. Nicoletti, P. Nanni, E. Dejana, and P. L. Lollini. 1993. Br. J. Cancer 68:862.
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Product Notes
The CD49d/VLA-4 (Catalog #AAA78664) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD49d/VLA-4a-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD49d/VLA-4 can be used in a range of immunoassay formats including, but not limited to, Blocking, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD49d/VLA-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49d/VLA-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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