IHC (Immunohiostchemistry)
(Dilution: IH 1/50 - 1/200Immunohistochemical analysis of CD16 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Mouse anti-Human CD16 Monoclonal Antibody | anti-CD16 antibody
Anti-CD16 Antibody
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
CD16, Antibody; Anti-CD16 Antibody; FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD16a; FCGR3B; CD16B; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIIIb; FcRIIIb; IgG Fc receptor III-1; CD16b; anti-CD16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of CD16 protein.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Applicable Applications for anti-CD16 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Cellular Location
Cell membrane; Single-pass type I membrane protein. Secreted. Note=Exists also as a soluble receptor
Tissue Location
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Dilution: IH 1/50 - 1/200Immunohistochemical analysis of CD16 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Dilution: IH 1/50 - 1/200Immunohistochemical analysis of CD16 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Dilution: WB 1/1000 - 1/2000Western blot analysis of CD16 expression in Jurkat (A), K562 (B) whole cell lysates.)
WB (Western Blot)
(Dilution: WB 1/1000 - 1/2000Western blot analysis of CD16 expression in Jurkat (A), K562 (B) whole cell lysates.)
Related Product Information for anti-CD16 antibody
Mouse monoclonal antibody to CD16
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
254
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III-A
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
NCBI Official Symbol
FCGR3A
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN
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Product Notes
The CD16 fcgr3a (Catalog #AAA291251) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD16 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD16 fcgr3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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