Mouse anti-Human CD158d Monoclonal Antibody | anti-CD158d antibody

Mouse Anti Human CD158d

Cat. Num. AAA50381

• Gene Names: KIR2DL4; G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Functional Assay, Flow Cytometry, Functional Assay
• Purity: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: CD158d, Antibody; Mouse Anti Human CD158d; CD158d antibody, mAb#33; anti-CD158d antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: mAb#33
• Purity/Purification: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG - Liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 377

• Applicable Applications for anti-CD158d antibody: WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), FA (Functional Assay), FCM/FACS (Flow Cytometry)
• Immunogen: NK3.3 cells and KIR2DL4-Ig fusion protein
• Preservative Stabilizers: <0.1% Sodium Azide (NaN3)
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE.; This product should be stored undiluted.

Application Data

(Surface Staining of human peripheral blood using Mouse anti Human CD158d:RPE)

Application Data

(Surface Staining of human peripheral blood using Mouse anti Human CD158d:APC)

Related Product Information for anti-CD158d antibody

Mouse anti Human CD158d, clone mAb#33, recognizes the extracellular portion of CD158d also known as Killer Cell Immunoglobulin-like Receptor, 2 domains, long cytoplasmic tail, 4 (KIR2DL4). CD158d is a 45 kDa natural killer (NK) cell marker, although cell surface expression and function depends on the genotype of particular individuals. As some individual have no detectable CD158d on the surface of resting NK cells. In some individuals surface levels of CD158d can increase upon stimulation by IL-2 (Kikuchi-Maki et a. 2003). The killer cell Ig-like receptor protein regulates the responsiveness of NK cells upon their contact with tumor cells, virus infected cells, cytokines and chemokines. CD158d is constitutively expressed by NK cells and recognizes major histocompatibility complex (MHC) class I molecules on target cells. The non-classical MHC class I molecule HLA-G is the only known ligand of CD158d (Rahagioakab & Long 1999).
CD158d contains two Ig domains and a single tyrosine based inhibition motif in the cytoplasmic domain. It also contains a charged arginine near the top of the transmembrane region which helps regulate the signal for cytotoxicity and cytokine secretion when engaged at the cell surface. These two features give CD158d both inhibitory and activating characteristics (Fauve & Long 2002).
This mAb#33 clone has been used in flow cytometry to examine CD158d levels upon infection of human extravillous cytotrophoblast cells with Toxoplasma gondii (Xu et al. 2013).

NCBI and Uniprot Product Information

• NCBI GI #: 325511396
• NCBI GeneID: 3805
• UniProt Accession #: Q99706
• NCBI Official Full Name: Killer cell immunoglobulin-like receptor 2DL4
• NCBI Official Synonym Full Names: killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4
• NCBI Official Symbol: KIR2DL4
• NCBI Official Synonym Symbols: G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL4
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL4
• UniProt Gene Name: KIR2DL4
• UniProt Synonym Gene Names: CD158D; KIR103AS; KIR-103AS