Application Data
((1ug/ml) staining in MCF7(A) cells lysate and (0.003ug/ml) staining in human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Mouse anti-Human Carcino Embryonic Antigen CEA Monoclonal Antibody | anti-CEA antibody
Carcino Embryonic Antigen CEA Monoclonal Antibody [CB30]
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A or G purified
Synonyms
Carcino Embryonic Antigen CEA, Antibody; Carcino Embryonic Antigen CEA Monoclonal Antibody [CB30]; anti-CEA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CB30
Purity/Purification
Protein A or G purified
Form/Format
Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CEA antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Full length native protein (purified)(Human)
Target Antigen
Carcino Embryonic Antigen CEA
Pubmed ID
24797403
Application Data
((1ug/ml) staining in MCF7(A) cells lysate and (0.003ug/ml) staining in human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Application Data
((1ug/ml) staining in MCF7(A) cells lysate and (0.003ug/ml) staining in human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Blue line: Flow cytometric analysis of MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)
FCM/FACS (Flow Cytometry)
(Blue line: Flow cytometric analysis of MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,795 Da
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
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Product Notes
The CEA ceacam5 (Catalog #AAA59542) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Carcino Embryonic Antigen CEA Monoclonal Antibody [CB30] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carcino Embryonic Antigen CEA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CEA ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carcino Embryonic Antigen CEA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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