ICC (Immunocytochemistry)
(ICC staining CaMK�� in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit CaMK Monoclonal Antibody | anti-CaMK antibody
CaMK Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
ICC (Immunocytochemistry)
(ICC staining CaMK�� in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CaMK�� in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CaMK�� in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CaMK�� in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CaMK�� in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CaMK�� in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMK�� antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of CaMK�� on different lysates using anti-CaMK�� antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: PC-12 Lane 3: SHG-44)
WB (Western Blot)
(Western blot analysis of CaMK�� on different lysates using anti-CaMK�� antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: PC-12 Lane 3: SHG-44)
NCBI and Uniprot Product Information
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Product Notes
The CaMK camk2b (Catalog #AAA30073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CaMK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CaMK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CaMK camk2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CaMK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is CaMK used as a marker for in cell signaling and neuroscience research?
CaMK (Calcium/Calmodulin-dependent Protein Kinase) serves as a marker for neuronal activity, synaptic signaling strength, and learning-associated molecular changes. Researchers use CaMK detection to study synaptic plasticity, long-term potentiation (LTP), memory formation, and assess neuronal activation states in normal and pathological conditions.
Can a CaMK monoclonal antibody be used to detect activated (phosphorylated) kinase?
Yes, phospho-CaMK monoclonal antibodies specifically recognize phosphorylated CaMK (particularly CaMKII phosphorylated at Thr286), representing the enzymatically active form. These antibodies enable researchers to directly measure CaMK activation status, distinguish between basal and activity-dependent phosphorylation, and track kinase activation dynamics in real-time.
Is CaMK expression linked to learning, memory, or synaptic plasticity studies?
Yes, CaMKII (the primary neuronal CaMK isoform) is critical for synaptic plasticity, long-term potentiation (LTP), and memory consolidation. Phosphorylated CaMKII is considered a molecular marker of recent neuronal activity and learning-associated synaptic strengthening, making CaMK antibodies essential tools for neuroscience research on memory and learning mechanisms.
Can CaMK antibody be used for immunofluorescence and Western blot applications?
Yes, CaMK monoclonal antibodies are validated for both immunofluorescence (enabling visualization of dendritic spine localization and subcellular CaMK distribution) and Western blotting (quantifying total and phosphorylated CaMK levels). These complementary techniques provide spatial and quantitative information about CaMK activity patterns.
What’s the difference between CaMK isoforms (CaMKII vs CaMKI/IV) in detection?
CaMKII (particularly alpha and beta subunits) is the predominant neuronal isoform, while CaMKI/IV is more ubiquitous. CaMKII-specific antibodies detect ~50-60 kDa subunits in brain tissue, whereas CaMKI/IV antibodies recognize distinct size variants. Isoform-specific antibodies enable the elective study of particular CaMK functions relevant to neuronal vs. non-neuronal contexts.
Is CaMK commonly studied in cardiac or neuronal tissue research?
Yes, CaMK (particularly CaMKIV) is extensively studied in cardiac tissue where it regulates contractility and arrhythmia susceptibility. However, CaMKII is the predominant neuronal isoform and is more frequently studied in neuroscience. This antibody's isoform specificity determines its primary research applications and tissue distribution patterns.
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