FCM/FACS (Flow Cytometry)
(a. Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).b. Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human ATGL Monoclonal Antibody | anti-ATGL antibody
ATGL antibody
FCM/FACS (Flow Cytometry)
(a. Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).b. Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM/FACS (Flow Cytometry)
(a. Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).b. Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with AAA48238 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
IF (Immunofluorescence)
(a. ICC/IF analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).b. ICC/IF analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).c. ICC/IF analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(a. ICC/IF analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).b. ICC/IF analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).c. ICC/IF analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(a. The A431 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.b. The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(a. The A431 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.b. The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
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Product Notes
The ATGL pnpla2 (Catalog #AAA48238) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATGL antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATGL can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ATGL pnpla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATGL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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