ICC (Immunocytochemistry)
(Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse Annexin A2 Monoclonal Antibody | anti-A2 antibody
Annexin A2 (C-terminal region) Antibody
Gene Names
Anxa2; ANX2
Reactivity
Human, Rat, Mouse, Chicken
Applications
Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, ELISA, Western Blot
Synonyms
Annexin A2, Antibody; Annexin A2 (C-terminal region) Antibody; Annexin A2 (C-terminal region); Annexin A2 M298; anti-A2 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M298
Specificity
This antibody detects a 36kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm.
Form/Format
Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-A2 antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).)
WB (Western Blot)
(Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).)
Related Product Information for anti-A2 antibody
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
References
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,893 Da
NCBI Official Full Name
annexin A2
NCBI Official Synonym Full Names
annexin A2
NCBI Official Symbol
Anxa2
NCBI Official Synonym Symbols
ANX2
NCBI Protein Information
annexin A2
UniProt Protein Name
Annexin A2
UniProt Gene Name
Anxa2
UniProt Synonym Gene Names
Anx2; PAP-IV
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Product Notes
The A2 anxa2 (Catalog #AAA71560) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Annexin A2 (C-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the A2 anxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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