WB (Western Blot)
( Western Blot using anti-Amyloid beta antibody 6E10 Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). frontal cortex and cerebellum lysate samples.)
amyloid beta Recombinant Antibody | A-beta recombinant antibody
Anti-amyloid beta [6E10]
Reactivity
Human
Applications
Immunoprecipitation, Western Blot, ELISA, Immunohistochemistry
Purity
PurifiedProtein A Affinity Purified
Synonyms
amyloid beta, Recombinant Antibody; Anti-amyloid beta [6E10]; amyloid beta-peptide; A-beta recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
6E10
Specificity
This antibody binds to amino acid residues 1-16 of beta amyloid.
Purity/Purification
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Concentration
1mg/mL (varies by lot)
Applicable Applications for A-beta recombinant antibody
IP (Immunoprecipitation), WB (Western Blot), ELISA, IHC (Immunohistochemistry)
Immunogen
Amyloid-beta peptide.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describesthe use of this antibody in a sandwich ELISA to detect amyloid protein.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
WB (Western Blot)
( Western Blot using anti-Amyloid beta antibody 6E10 Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). frontal cortex and cerebellum lysate samples.)
WB (Western Blot)
( Western Blot using anti-Amyloid beta antibody 6E10 Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). frontal cortex and cerebellum lysate samples.)
IHC (Immunohistochemistry)
( Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.)
IHC (Immunohistochemistry)
( Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.)
Product Categories/Family for A-beta recombinant antibody
References
Pirttilä et al. J Neurol Sci. 1994 Dec 1;127(1):90-5. PMID:7699397
NCBI and Uniprot Product Information
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Product Notes
The A-beta (Catalog #AAA71978) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-amyloid beta [6E10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's amyloid beta can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the A-beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "amyloid beta, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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