FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit alpha Tubulin 4A Monoclonal Antibody | anti-TUBA4A antibody
alpha Tubulin 4A Antibody
IHC: 1:100-1:200
ICC: 1:100-1:200
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1, 000 dilution. Positive control�� Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12)
WB (Western Blot)
(Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1, 000 dilution. Positive control�� Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12)
NCBI and Uniprot Product Information
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Product Notes
The TUBA4A tuba4a (Catalog #AAA30369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Tubulin 4A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Tubulin 4A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). WB: 1:500-1:2000 IHC: 1:100-1:200 ICC: 1:100-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TUBA4A tuba4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin 4A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is alpha-Tubulin 4A?
Alpha-Tubulin 4A is a protein inside a cell that helps build microtubules, which give cells shape and help with cell division and transport inside the cell. Researchers study it to learn more about how cells work and stay healthy.
Is alpha-Tubulin 4A suitable as a loading control for Western blot?
Yes, alpha-tubulin 4A is suitable as a loading control for Western blot experiments, because tubulin proteins are usually present at steady levels. It is a structural protein, so researchers should always check its stability in their experiment first.
How is alpha-Tubulin 4A different from other alpha-tubulin isoforms?
Alpha-Tubulin 4A is one specific form of alpha-tubulin. While all alpha-tubulin isoforms form microtubules. They have tiny differences in their structure and amounts, which affect where they're made in cells.
Can an alpha-Tubulin 4A antibody be used to visualize microtubules by immunofluorescence?
Yes, the alpha-Tubulin 4A antibody is suitable for immunofluorescence. They bind to microtubules, lighting them up under a fluorescence microscope to show the cell's internal network.
Does alpha-Tubulin 4A expression remain stable across different cell types?
Yes, alpha-Tubulin 4A is generally expressed in many cell types because it is part of the cell structure. However, its levels can shift with cell type, growth, or treatments. Researchers should always check and confirm its stability before using it in the experiment.
Is alpha-Tubulin 4A commonly used as a housekeeping protein in experiments?
Yes, alpha-Tubulin 4A is commonly used as a housekeeping protein in experiments, specifically in Western blots. Tubulins are common for this, but it's not as widely used as total alpha-tubulin. Its suitability depends on the experimental setup and sample type.
What is alpha Tubulin 4A used as a marker for in cell biology studies?
Alpha Tubulin 4A (TUBA4A) is a core component of microtubules forming the cellular cytoskeleton. It serves as a robust housekeeping/reference protein marker in cell biology for assessing microtubule organization, cytoskeletal integrity, neurite outgrowth, and cell cycle progression in various experimental conditions.
Can an alpha Tubulin 4A antibody be used as a loading control in Western blots?
Yes, TUBA4A (~50 kDa) is one of the most reliable housekeeping proteins for Western blot loading controls. Its expression remains relatively stable across diverse tissues and experimental conditions, making it ideal for normalizing protein sample quantities and correcting for gel-loading variations in quantitative blot analysis.
Is alpha Tubulin 4A suitable for immunofluorescence to visualize microtubule networks?
Absolutely. This antibody specifically labels microtubule networks in fixed cells and tissues via IF, enabling visualization of microtubule organization, dynamics during cell division, and alterations in microtubule structure associated with neurodegenerative diseases, developmental processes, or drug treatments affecting microtubule stability.
How does alpha Tubulin 4A differ from general alpha-tubulin antibodies?
TUBA4A is one of six alpha-tubulin isoforms with specific tissue and subcellular distributions. While general alpha-tubulin antibodies recognize all isoforms, TUBA4A-specific antibodies provide isoform-specific detection, useful for studying isoform-selective functions or tissue-restricted expression patterns relevant to particular disease contexts.
Does alpha Tubulin 4A expression remain consistent across different cell types?
TUBA4A expression is generally stable across most cell types and tissues, making it reliable for loading control applications. However, expression is notably low in adipose tissue, requiring alternative loading controls for fat tissue analyses. In most other tissues, TUBA4A maintains consistent, abundant expression under standard conditions.
Is alpha Tubulin 4A commonly used as a housekeeping protein in research assays?
Yes, TUBA4A is one of the three most widely used housekeeping proteins alongside beta-actin and GAPDH. It is the preferred loading control in many neuroscience and cell biology applications, offers superior consistency in certain experimental conditions compared to actin, and is recommended for studies involving neuronal cells.
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