ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit alpha Synuclein Monoclonal Antibody | anti-SNCA antibody
alpha Synuclein (Phospho-S129) Antibody
ICC: 1:50-1:200
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-alpha Synuclein (S129) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.)
NCBI and Uniprot Product Information
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Product Notes
The SNCA snca (Catalog #AAA29788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Synuclein (Phospho-S129) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Synuclein can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the SNCA snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Synuclein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is Rabbit alpha-Synuclein Monoclonal Antibody used for?
This antibody is used to detect alpha-synuclein, a protein primarily found in the brain's presynaptic nerve terminals. It is a key tool in neurodegenerative disease research, specifically for studying the protein's role in synaptic function and its abnormal aggregation in conditions like Parkinson's disease and Lewy body dementia.
How does alpha-Synuclein antibody help in neurological research?
In neurological research, the alpha-synuclein antibody helps visualize the formation of Lewy bodies and Lewy neurites. These are pathological hallmarks of synucleinopathies. By using this antibody, researchers can map the distribution of alpha-synuclein aggregates across different brain regions, providing insights into disease progression and the mechanisms of neuronal loss and synaptic dysfunction.
Which techniques can detect alpha-Synuclein with this antibody?
This antibody is versatile and can detect alpha-synuclein using techniques such as Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry. It is effective for analyzing both human tissue samples and animal models, allowing for the quantification of protein levels and the localization of synuclein within specific cellular compartments.
What are the benefits of using a monoclonal alpha-Synuclein antibody?
The monoclonal nature of this AAA Biotech antibody offers high specificity for the alpha isoform of synuclein, minimizing cross-reactivity with other proteins. It provides excellent reproducibility and batch-to-batch consistency, which is vital for long-term studies. Monoclonals also tend to produce cleaner results with lower background noise in imaging applications like immunofluorescence.
How should this antibody be stored and handled?
The antibody should be stored at -20°C to maintain its activity. For short-term use, storage at 4°C for up to one month is often acceptable. It is recommended to avoid repeated freeze-thaw cycles by creating small aliquots. If the antibody is conjugated to a fluorophore, it must be protected from light to prevent photobleaching.
What should researchers consider when selecting an alpha-Synuclein antibody?
Researchers should consider whether they need to detect monomeric, phosphorylated, or aggregated forms of alpha-synuclein. Additionally, checking species reactivity is essential, as some antibodies are optimized for human, mouse, or rat samples. AAA Biotech provides detailed validation data to help researchers select the most appropriate clone for their specific experimental goals and sample types.
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