FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-alpha-gal epitope antibody m86 (AAA72085). Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of m86 (AAA72085-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Human alpha-gal epitope Monoclonal Antibody | anti-alpha-galepitope antibody
Anti-alpha-gal epitope [m86]
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-alpha-gal epitope antibody m86 (AAA72085). Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of m86 (AAA72085-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-alpha-gal epitope antibody m86 (AAA72085). Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of m86 (AAA72085-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
WB (Western Blot)
(Western Blot using anti-alpha-gal epitope antibody m86 (AAA72085). rat spleen(A) and pig spleen(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of m86 (AAA72085-1.1) at 0.3ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-alpha-gal epitope antibody m86 (AAA72085). rat spleen(A) and pig spleen(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of m86 (AAA72085-1.1) at 0.3ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-alpha-gal epitope antibody m86 (AAA72085). RAW264.7 cells were stained with anti-unknown IgG antibody or the mouse IgG-chimeric version of m86 (AAA72085-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-alpha-gal epitope antibody m86 (AAA72085). RAW264.7 cells were stained with anti-unknown IgG antibody or the mouse IgG-chimeric version of m86 (AAA72085-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of RAW264.7 cells using anti-alpha-gal epitope antibody (AAA72085) m86. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric mouse IgG version of m86 (AAA72085-1.1) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72085-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of RAW264.7 cells using anti-alpha-gal epitope antibody (AAA72085) m86. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric mouse IgG version of m86 (AAA72085-1.1) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72085-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Rabbit red cells, on which alpha-gal is the major carbohydrate epitope, were injected into alpha 1,3 galactosyltransferase knock-out mice.
Note on publication: Describes the generation and characterization of anti-gal epitope antibodies raised in alpha 1,3 galactosyltransferase knock-out mice.
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Product Notes
The alpha-galepitope (Catalog #AAA72085) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-alpha-gal epitope [m86] reacts with Species independent and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-gal epitope can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the alpha-galepitope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-gal epitope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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