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FCM/FACS (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.)
Mouse anti-Human ALOX15 / 15-Lipoxygenase Monoclonal Antibody | anti-ALOX15 antibody
Anti-ALOX15 / 15-Lipoxygenase Antibody (clone 7H6) IHC-plus
Gene Names
ALOX15; 12-LOX; 15LOX-1; 15-LOX-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein A/G purified
Synonyms
ALOX15 / 15-Lipoxygenase, Antibody; Anti-ALOX15 / 15-Lipoxygenase Antibody (clone 7H6) IHC-plus; Mouse Monoclonal [clone 7H6] (IgG2b) to Human ALOX15 / 15-Lipoxygenase; Human ALOX15 / 15-Lipoxygenase; 15-lipoxygenase; 15-LOX; 15-lipooxygenase-1; 15-LO-1; 15LOX-1; 15-LOX-1; LOG15; Arachidonate 15-lipoxygenase; anti-ALOX15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H6
Specificity
Human 15-LOX / ALOX15
Purity/Purification
Protein A/G purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1.21 mg/ml (varies by lot)
Sequence Length
662
Applicable Applications for anti-ALOX15 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
ALOX15 / 15-Lipoxygenase antibody was raised against full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.)
FCM/FACS (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.)
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.)
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.)
IF (Immunofluorescence)
(Anti-ALOX15 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15.)
IF (Immunofluorescence)
(Anti-ALOX15 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15.)
IHC (Immunohiostchemistry)
(Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-ALOX15 antibody
ALOX15 Antibody, 15-lipoxygenase Antibody, 15-LOX Antibody, 15-lipooxygenase-1 Antibody, 15-LO-1 Antibody, 15LOX-1 Antibody, 15-LOX-1 Antibody, LOG15 Antibody, Arachidonate 15-lipoxygenase Antibody Description: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,108 Da
NCBI Official Full Name
arachidonate 15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15
NCBI Official Synonym Symbols
12-LOX; 15LOX-1; 15-LOX-1
NCBI Protein Information
arachidonate 15-lipoxygenase
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Gene Name
ALOX15
UniProt Synonym Gene Names
LOG15; 12-LOX
UniProt Entry Name
LOX15_HUMAN
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Product Notes
The ALOX15 alox15 (Catalog #AAA52585) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALOX15 / 15-Lipoxygenase Antibody (clone 7H6) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX15 / 15-Lipoxygenase can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ALOX15 alox15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX15 / 15-Lipoxygenase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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