Mouse anti-Human ACY1 Monoclonal Antibody | anti-ACY1 antibody

ACY1 antibody

Cat. Num. AAA48147

• Gene Names: ACY1; ACY-1; ACY1D; HEL-S-5
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: By protein-A affinity chromatography
• Synonyms: ACY1, Antibody; ACY1 antibody; Aminoacylase 1; ACY1D; ACYLASE; anti-ACY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: AT1E2
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 408

• Applicable Applications for anti-ACY1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Antigen Species: Human
• Immunogen: Recombinant human ACY1 (1-408aa) purified from E. coli
• Clone Note: Anti-human ACY1 mAb, clone AT1E2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACY1 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

IF (Immunofluorescence)

(ICC/IF analysis of ACY1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACY1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot)

(Fig 1.: The cell lysates of Jurkat, HepG2, Hela, A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Recombinant Human ACY1 (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-ACY1 antibody

Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.

Product Categories/Family for anti-ACY1 antibody

Enzymes & Proteases; Signal Transduction; Metabolism

References

Cook R.M., et al. (1993) J Biol Chem 268 (23): 17010-17017; Lindner H.A., et al. (2003) J Biol Chem 278 (45): 44496-44504

NCBI and Uniprot Product Information

• NCBI GI #: 4501901
• NCBI GeneID: 95
• NCBI Accession #: NP_000657
• NCBI GenBank Nucleotide #: NM_000666.2
• UniProt Accession #: Q03154; C9J6I6; C9J9D8; C9JWD4
• Molecular Weight: 42,195 Da
• NCBI Official Full Name: aminoacylase-1 isoform a
• NCBI Official Synonym Full Names: aminoacylase 1
• NCBI Official Symbol: ACY1
• NCBI Official Synonym Symbols: ACY-1; ACY1D; HEL-S-5
• NCBI Protein Information: aminoacylase-1
• UniProt Protein Name: Aminoacylase-1
• UniProt Gene Name: ACY1
• UniProt Synonym Gene Names: ACY-1