Mouse actin alpha-skeletal Monoclonal Antibody | anti-ACTA1 antibody

Mouse anti actin alpha-skeletal

Cat. Num. AAA77514

• Gene Names: ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• Reactivity: Caprine, Human, Rabbit, Rat, Swine.
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunoblot, Western Blot, ELISA
• Synonyms: actin alpha-skeletal, Antibody; Mouse anti actin alpha-skeletal; anti-ACTA1 antibody

• Host: Mouse
• Reactivity: Caprine, Human, Rabbit, Rat, Swine.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3B3
• Specificity: Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-ACTA1 antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IB (Immunoblot), ELISA
• Source Note: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
• Reactivity Note: The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
• Product Type: Primary Antibody
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Application Data

Application Data

(Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250))

Related Product Information for anti-ACTA1 antibody

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.

Product Categories/Family for anti-ACTA1 antibody

Cytoskeletal; Cardiovascular; Cell differentiation; Cytoskeleton; Stem cell research

References

Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.

NCBI and Uniprot Product Information

• NCBI GI #: 4501881
• NCBI GeneID: 58
• NCBI Accession #: NP_001091.1
• NCBI GenBank Nucleotide #: NM_001100.3
• UniProt Accession #: P68133; P02568; P99020; Q5T8M9
• NCBI Official Full Name: actin, alpha skeletal muscle
• NCBI Official Synonym Full Names: actin, alpha 1, skeletal muscle
• NCBI Official Symbol: ACTA1
• NCBI Official Synonym Symbols: ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• NCBI Protein Information: actin, alpha skeletal muscle; nemaline myopathy type 3
• UniProt Protein Name: Actin, alpha skeletal muscle
• UniProt Gene Name: ACTA1
• UniProt Synonym Gene Names: ACTA
• UniProt Entry Name: ACTS_HUMAN