Monkey Pulmonary Surfactant-Associated Protein D (SFTPD) ELISA Kit | SFTPD elisa kit

Monkey Pulmonary Surfactant-Associated Protein D (SFTPD) ELISA Kit

Cat. Num. AAA247502

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Reactivity: Monkey
• Synonyms: Pulmonary Surfactant-Associated Protein D (SFTPD); N/A; Monkey Pulmonary Surfactant-Associated Protein D (SFTPD) ELISA Kit; SFTPD elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPD. No significant cross-reactivity or interference between SPD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPD and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 375
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for SFTPD elisa kit

Principle of the Assay: SPD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SPD antibody and an SPD-HRP conjugate. The assay sample and buffer are incubated together with SPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPD concentration since SPD from samples and SPD-HRP conjugate compete for the anti-SPD antibody binding site. Since the number of sites is limited, as more sites are occupied by SPD from the sample, fewer sites are left to bind SPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPD concentration in each sample is interpolated from this standard curve.

Intended Uses: This SPD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SPD. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for SFTPD elisa kit

Immunology; Microbiology; Signal Transduction; Cancer; Cardiovascular; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 61699226
• NCBI GeneID: 6441
• NCBI Accession #: NP_003010.4
• NCBI GenBank Nucleotide #: NM_003019.5
• UniProt Accession #: P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
• Molecular Weight: 37,728 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D