Application Data
(Recombinant Human interleukin-11 (AAA50427) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains.)
Interleukin-11 Recombinant Protein | IL-11 recombinant protein
Recombinant Human Interleukin-11
Gene Names
IL11; AGIF; IL-11
Applications
ELISA
Purity
Recombinant protein expressed in E Coli and purified by ion exchange chromatography>=90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Synonyms
Interleukin-11; N/A; Recombinant Human Interleukin-11; IL-11; IL-11 recombinant protein
Host
E Coli
Purity/Purification
Recombinant protein expressed in E Coli and purified by ion exchange chromatography
>=90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
>=90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Form/Format
50 mM Tris-HCl, pH8.0, 0.15 M NaCl
1.0% Trehalose
0.05% Tween80
Purified recombinant protein - lyophilized
1.0% Trehalose
0.05% Tween80
Purified recombinant protein - lyophilized
Concentration
100ug/ml after reconstitution (varies by lot)
Applicable Applications for IL-11 recombinant protein
ELISA
Target Species
Human
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100ug/ml by adding 100ul ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Lab recommends that the vial is gently mixed after reconstitution. Do not vortex.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Lab recommends that the vial is gently mixed after reconstitution. Do not vortex.
Preparation and Storage
Prior to reconstitution store at -70 degree C. Following reconstitution store at -80 degree C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Recombinant Human interleukin-11 (AAA50427) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains.)
Application Data
(Recombinant Human interleukin-11 (AAA50427) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains.)
ELISA
(Sandwich ELISA analysis of human IL-11 using Mouse anti Human interleukin-11 as a capture reagent and biotinylated Mouse anti Human interleukin-11 as a detection reagent with recombinant Human interleukin-11 (AAA50427) as the antigen.)
ELISA
(Sandwich ELISA analysis of human IL-11 using Mouse anti Human interleukin-11 as a capture reagent and biotinylated Mouse anti Human interleukin-11 as a detection reagent with recombinant Human interleukin-11 (AAA50427) as the antigen.)
Related Product Information for IL-11 recombinant protein
Recombinant Human interleukin-11
Interleukin-11 (IL-11), also known as AGIF belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013).
This recombinant Human interleukin-11 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 as a capture antibody and biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 as the detection antibody.
Interleukin-11 (IL-11), also known as AGIF belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013).
This recombinant Human interleukin-11 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 as a capture antibody and biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 as the detection antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,429 Da
NCBI Official Full Name
interleukin-11 isoform 2
NCBI Official Synonym Full Names
interleukin 11
NCBI Official Symbol
IL11
NCBI Official Synonym Symbols
AGIF; IL-11
NCBI Protein Information
interleukin-11; oprelvekin; adipogenesis inhibitory factor
UniProt Protein Name
Interleukin-11
UniProt Gene Name
IL11
UniProt Synonym Gene Names
IL-11; AGIF
UniProt Entry Name
IL11_HUMAN
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Product Notes
The IL-11 il11 (Catalog #AAA50427) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin-11 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IL-11 il11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin-11, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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