Standard Curve (Sample)
Interleukin-9 ELISA Kit | IL-9 elisa kit
Interleukin-9 (IL-9)
Gene Names
IL9; P40; HP40; IL-9
Synonyms
Interleukin-9; N/A; Interleukin-9 (IL-9); IL-9 elisa kit
Specificity
This sandwich ELISA can detect both natural and recombinant human IL-9. This kit exhibits no significant cross-reactivity with human IL-1alpha, IL-1beta, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, G-CSF, GM-CSF, TGF-beta1, TNF-alpha, TNF-beta, MCAF, MCP-3, bFGF, EGF, IFN-gamma.
Sequence Length
144
Samples
Serum, plasma, Cell Culture Supernatant, and other biological fluids
Assay Type
Sandwich
Sensitivity
The minimum detectable dose of human IL-9 was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-9 calculated from calibrate I diluted standard curve was <0.343pg/mL.
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by replicates of eight in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by replicates on 8 different assays.
Intended Uses
This Human Interleukin 9 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 9 (IL-9) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-9 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-9 present is bound by the immobilized antibody. After washing away unbound substances, a biotin-linked antibody specific for IL- 9 is added to the wells. Following a wash to remove unbound biotin labelled antibody, an avidin-HRP conjugate is added to each wells. Avidin binds to biotin labelled antibody with high affinity. After TMB substrate solution is added to the wells, colour will develop in proportion to the amount of IL-9 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-9 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-9 concentration (pg/mL). The concentration of IL-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background: Interleukin 9(IL-9) was initially discovered as a 40KD glycoprotein with T-cell growth factor activity in mice (1). Human IL-9 gene was identified based on its remarkable homogeneity with the gene of mouse IL-9 (2) and was mapped to chromosome 5. Human IL-9 has been demonstrated to be a stimulator for hematopoiesis and be associated with human malignant lymphoma (3, 4, 5, 6). In addition, IL-9 and IL-9 receptor expression was found to be implicated with autoimmune and allergic diseases (7, 8). IL-9 has been studied as a therapeutic target for asthma (7, 9) because of its implication with the disease. IL-9 can be produced by Th2 cells under the stimulation of TGF-beta and IL-4 and has been shown to play a role in the differentiation of Th17 cells and Treg function (10, 11). In recent years, IL-9 has received renewed attention because a unique type of innate lymphoid cells has been identified as the main cell type that expresses IL-9 in vivo and the IL-9 secreting cells present in skin appear to be mediate inhibition of melanoma growth in mice (12,13,14,15). The same IL-9 secreting cells have been identified in human and the cells are currently evaluated as a new tumour treatment strategy.
Background: Interleukin 9(IL-9) was initially discovered as a 40KD glycoprotein with T-cell growth factor activity in mice (1). Human IL-9 gene was identified based on its remarkable homogeneity with the gene of mouse IL-9 (2) and was mapped to chromosome 5. Human IL-9 has been demonstrated to be a stimulator for hematopoiesis and be associated with human malignant lymphoma (3, 4, 5, 6). In addition, IL-9 and IL-9 receptor expression was found to be implicated with autoimmune and allergic diseases (7, 8). IL-9 has been studied as a therapeutic target for asthma (7, 9) because of its implication with the disease. IL-9 can be produced by Th2 cells under the stimulation of TGF-beta and IL-4 and has been shown to play a role in the differentiation of Th17 cells and Treg function (10, 11). In recent years, IL-9 has received renewed attention because a unique type of innate lymphoid cells has been identified as the main cell type that expresses IL-9 in vivo and the IL-9 secreting cells present in skin appear to be mediate inhibition of melanoma growth in mice (12,13,14,15). The same IL-9 secreting cells have been identified in human and the cells are currently evaluated as a new tumour treatment strategy.
Product Categories/Family for IL-9 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,909 Da
NCBI Official Full Name
interleukin-9
NCBI Official Synonym Full Names
interleukin 9
NCBI Official Symbol
IL9
NCBI Official Synonym Symbols
P40; HP40; IL-9
NCBI Protein Information
interleukin-9; T-cell growth factor p40; cytokine P40; homolog of mouse T cell and mast cell growth factor 40; p40 T-cell and mast cell growth factor; p40 cytokine
UniProt Protein Name
Interleukin-9
UniProt Gene Name
IL9
UniProt Entry Name
IL9_HUMAN
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Product Notes
The IL-9 il9 (Catalog #AAA77887) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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