Application Data
(This assay recognizes recombinant Human TREM2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human TREM-2/TREM2 ELISA Kit | TREM-2/TREM2 elisa kit
Human TREM-2/TREM2 ELISA Kit
Reactivity
Human
Synonyms
TREM-2/TREM2; N/A; Human TREM-2/TREM2 ELISA Kit; TREM-2 ELISA Kit, Human; triggering receptor expressed on myeloid cells 2; Trem2a ELISA Kit, Human; Trem2b ELISA Kit, Human; Trem2c ELISA Kit, Human; TREM-2/TREM2 elisa kit
Reactivity
Human
Specificity
The kit has been verified by high purity Human TREM-2/TREM2 recombinant protein.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Recombinant protein
Detection Range
78.13-5000pg/mL
Sensitivity
33.48pg/mL
Conjugation
HRP
Interference
Preparations of human TREML2 at 50ng/mL in a mid-range recombinant human TREM2 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes recombinant Human TREM2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes recombinant Human TREM2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for TREM-2/TREM2 elisa kit
This TREM-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM-2 protein in Recombinant protein. It contains recombinant Human TREM-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Triggering receptor expressed on myeloid cells 2 (TREM2) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.
Background: Triggering receptor expressed on myeloid cells 2 (TREM2) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.
References
Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995.
Paloneva, J. et al., 2002, Am. J. Hum. Genet. 71:656-662.
Prada, I. et al., 2006, Neuroscience. 140 (4): 1139-48.
Neumann, H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9.
Thrash, JC. et al., 2009, Neurochem Res. 34 (1): 38-45.
Paloneva, J. et al., 2002, Am. J. Hum. Genet. 71:656-662.
Prada, I. et al., 2006, Neuroscience. 140 (4): 1139-48.
Neumann, H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9.
Thrash, JC. et al., 2009, Neurochem Res. 34 (1): 38-45.
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Product Notes
The Human TREM-2/TREM2 (Catalog #AAA258419) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258419 ELISA Kit recognizes Human TREM-2/TREM2. It is sometimes possible for the material contained within the vial of "TREM-2/TREM2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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