Standard Curve (Sample)
Human Neurogenin 3 (NEUROG3) ELISA Kit | NEUROG3 elisa kit
Human Neurogenin 3 (NEUROG3) ELISA Kit
Gene Names
NEUROG3; ngn3; Atoh5; NGN-3; Math4B; bHLHa7
Reactivity
Human
Synonyms
Neurogenin 3 (NEUROG3); N/A; Human Neurogenin 3 (NEUROG3) ELISA Kit; Math4B; Atoh5; ngn3; bHLHa7; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5; NEUROG3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NEUROG3. No significant cross-reactivity or interference between NEUROG3 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEUROG3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEUROG3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unused kit: The whole kit could be stored at -20oC in shelf life, while up to one month at 4oC. For experiment convenience, reagents could also be stored seperately, Standard, Detection Reagent A, Detection Reagent B and 96-well strip plate should be stored at -20oC while the others could be at 4 oC.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NEUROG3 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NEUROG3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NEUROG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEUROG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEUROG3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEUROG3 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEUROG3 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for NEUROG3 elisa kit
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Product Notes
The Human NEUROG3 neurog3 (Catalog #AAA152360) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA152360 ELISA Kit recognizes Human NEUROG3. It is sometimes possible for the material contained within the vial of "Neurogenin 3 (NEUROG3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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