Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit | KAZALD1 elisa kit

Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit

Cat. Num. AAA66045

• Gene Names: KAZALD1; BONO1; FKSG28; FKSG40; IGFBP-rP10
• Reactivity: Human
• Synonyms: Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1); N/A; Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit; DFNA14; DFNA38; DFNA6; DIDMOAD; WFRS; WFS; Wolframin; KAZALD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KAZALD1. No significant cross-reactivity or interference between KAZALD1 and analogues was observed.
• Sequence Length: 166
• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KAZALD1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KAZALD1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for KAZALD1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KAZALD1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KAZALD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KAZALD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KAZALD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KAZALD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 985482517
• NCBI GeneID: 81621
• NCBI Accession #: NP_001306232.1
• NCBI GenBank Nucleotide #: NM_001319303.1
• UniProt Accession #: Q96I82; Q6ZMB1; Q9BQ73; D3DR74
• Molecular Weight: 26,525 Da
• NCBI Official Full Name: kazal-type serine protease inhibitor domain-containing protein 1 isoform b
• NCBI Official Synonym Full Names: Kazal type serine peptidase inhibitor domain 1
• NCBI Official Symbol: KAZALD1
• NCBI Official Synonym Symbols: BONO1; FKSG28; FKSG40; IGFBP-rP10
• NCBI Protein Information: kazal-type serine protease inhibitor domain-containing protein 1
• UniProt Protein Name: Kazal-type serine protease inhibitor domain-containing protein 1
• UniProt Gene Name: KAZALD1