Standard Curve (Sample)
IFN gamma elisa kit
Human IFN gamma HS ELISA Kit
Standard Curve (Sample)
Standard Curve (Sample)
Principle of the Assay: The IFNgamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFNgamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNgamma concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNgamma antigen and a biotinylated monoclonal antibody specific for IFNgamma are successively incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFNgamma present in the samples.
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Product Notes
The Human. No cross reactivity with other human cytokines. IFN gamma ifng (Catalog #AAA13472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13472 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. IFN gamma. It is sometimes possible for the material contained within the vial of "IFN gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What does an IFN-gamma ELISA test measure?
An IFN-γ (interferon-gamma) ELISA measures soluble IFN-γ protein concentration in biological samples. IFN-γ is a key Th1 cytokine produced by activated T cells and natural killer (NK) cells. The ELISA uses a sandwich immunoassay format with capture and detection antibodies specific for distinct IFN-γ epitopes.
How much does an IFN-gamma ELISA kit typically cost?
IFN-gamma ELISA kit costs vary widely by manufacturer and format, typically ranging from $300–$600 USD for a 96-well kit. Pricing depends on sensitivity, format (single-wash vs. standard), included reagents, and antibody quality. Contact AAA Biotech directly for current pricing on their specific IFN-gamma ELISA product.
What conditions or immune responses is IFN-gamma a marker for?
IFN-γ is a marker of Th1-mediated cellular immunity and is elevated in response to intracellular pathogens (viruses, bacteria, parasites), autoimmune diseases, transplant rejection, and certain cancers. It is also used to assess vaccine responses and immune competence and is a key biomarker in TB infection screening (e.g., QuantiFERON gold tests).
Can an ELISA test be performed at home or only in a laboratory?
ELISA tests require specialized laboratory equipment (microplate reader, incubator, and washer) and should be performed in a clinical or research laboratory by trained personnel. Home-based testing is not feasible for standard ELISA formats; however, point-of-care rapid assays (not traditional ELISA) can be done in clinical settings or near-patient environments.
Why is IFN-gamma commonly measured in immune response studies?
IFN-γ is the prototypical Th1 cytokine and a central regulator of macrophage activation, antigen presentation, and antimicrobial/antitumor immunity. It is measured to assess T cell immune function, evaluate vaccine efficacy, diagnose intracellular infections (TB), monitor immune reconstitution in HIV/AIDS, and study immune activation in clinical trials.
What sample types are used for IFN-gamma ELISA testing?
Compatible sample types include cell culture supernatants (from stimulated PBMCs or T cells), serum, plasma (EDTA, heparin, citrate), and tissue lysates. Cell culture supernatants from antigen-stimulated PBMCs are most commonly used in research, while serum/plasma are used in clinical diagnostics and vaccine response studies.
How accurate is an IFN-gamma ELISA test?
IFN-γ ELISA shows high accuracy and reproducibility with a typical sensitivity of 10–470 pg/mL and a dynamic range of 15–15,000 pg/mL, depending on the kit format. Accuracy is improved by using quality controls, standard curves, proper sample preparation, and standardized protocols. Inter-assay variability is typically <15% for validated kits.
How long does an IFN-gamma ELISA test take to get results?
Standard IFN-γ ELISA protocols require 4–5 hours from sample addition to final reading. Rapid, single-wash ELISA formats can reduce assay time to 90 minutes. Results are typically available the same day, with some automated platforms providing results within 2–3 hours.
What is the difference between IFN-gamma ELISA and rapid tests?
IFN-γ ELISA is a sandwich immunoassay requiring 90 minutes to 5 hours and providing quantitative results (pg/mL). Rapid tests (e.g., lateral flow assays) are qualitative or semi-quantitative, completed in 15–30 minutes, but with lower sensitivity and dynamic range. ELISA remains the gold standard for accurate quantification.
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