Human CXCL5/ENA-78 ELISA Kit | CXCL5/ENA-78 elisa kit

Human CXCL5/ENA-78 PicoKine Quick ELISA Kit

Cat. Num. AAA128070

• Gene Names: CXCL5; SCYB5; ENA-78
• Reactivity: Human
• Applications: ELISA
• Synonyms: CXCL5/ENA-78; N/A; Human CXCL5/ENA-78 PicoKine Quick ELISA Kit; CXCL5/ENA-78 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma(heparin, EDTA, citrate)
• Assay Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: A37-N114
• Anticoagulant: heparin, EDTA or citrate
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CXCL5/ENA-78 elisa kit: ELISA
• Kit Components: Description/Quantity/Volume; Anti-tag Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells; Standard/2, 10 ng/tube×2; Antibody A/1/3ml; Antibody B/1/3ml; Sample Diluent/1/15ml; TBS-T Wash Buffer (25x)/1/12ml; Color Developing Reagent (TMB)/1/10ml; Stop Solution/1/10ml; Adhesive plate sealers/2/Piece
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Automated plate washer (optional); Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.; Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
• Sample Data:

  Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
  O.D.	0.022	0.161	0.306	0.614	1.143	1.877	2.081	2.097

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	66	270	964	61	293	970
  Standard deviation	2.77	18.09	74.22	3.53	22.26	82.45
  CV (%)	4.2%	6.7%	7.7%	5.8%	7.6%	8.5%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	77	65	68	69	4.74	6.8%
  Sample 2	270	257	287	288	275	12.85	4.6%
  Sample 3	964	912	900	813	897	54.26	6%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human CXCL5/ENA-78 PicoKine Quick ELISA Kit standard curve)

Related Product Information for CXCL5/ENA-78 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CXCL5/ENA-78 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 471243
• NCBI GeneID: 6374
• UniProt Accession #: P42830; Q96QE1
• Molecular Weight: 11,972 Da
• NCBI Official Full Name: ENA-78
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: CXCL5
• NCBI Official Synonym Symbols: SCYB5; ENA-78
• NCBI Protein Information: C-X-C motif chemokine 5; neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; epithelial-derived neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating pept
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: CXCL5
• UniProt Synonym Gene Names: ENA78; SCYB5
• UniProt Entry Name: CXCL5_HUMAN