Standard Curve (Sample)
(Human Complement C2 PicoKine ELISA Kit Standard Curve)
Human Complement C2 ELISA Kit | C2 elisa kit
Human Complement C2 ELISA Kit PicoKine
Gene Names
C2; CO2; ARMD14
Reactivity
Human
Applications
ELISA
Synonyms
Complement C2; N/A; Human Complement C2 ELISA Kit PicoKine; C2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human C2
Samples
Cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<20 pg/ml
Applicable Applications for C2 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human Complement C2 PicoKine ELISA Kit Standard Curve)
Standard Curve (Sample)
(Human Complement C2 PicoKine ELISA Kit Standard Curve)
Related Product Information for C2 elisa kit
Background: Complement C2 is a protein that in humans is encoded by the C2 gene. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.
Principle of the Assay: The Picokine™ Human C2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human C2 with a 96-well strip plate that is pre-coated with antibody specific for C2. The detection antibody is a biotinylated antibody specific for C2. The capture antibody is monoclonal antibody from mouse and the detection antibody is biotinylated polyclonal antibody from goat. The kit includes Human C2 protein as standards. To measure Human C2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human C2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human C2 in the sample.
Principle of the Assay: The Picokine™ Human C2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human C2 with a 96-well strip plate that is pre-coated with antibody specific for C2. The detection antibody is a biotinylated antibody specific for C2. The capture antibody is monoclonal antibody from mouse and the detection antibody is biotinylated polyclonal antibody from goat. The kit includes Human C2 protein as standards. To measure Human C2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human C2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human C2 in the sample.
Product Categories/Family for C2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,444 Da
NCBI Official Full Name
complement C2 isoform 1 preproprotein
NCBI Official Synonym Full Names
complement component 2
NCBI Official Symbol
C2
NCBI Official Synonym Symbols
CO2; ARMD14
NCBI Protein Information
complement C2
UniProt Protein Name
Complement C2
UniProt Gene Name
C2
UniProt Entry Name
CO2_HUMAN
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Product Notes
The Human C2 c2 (Catalog #AAA126690) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126690 ELISA Kit recognizes Human C2. AAA Biotech's Complement C2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the C2 c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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