Standard Curve (Sample)
(Fig.1. Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) Standard Curve.)
Aminoacyl Trna Synthase Complex-Interacting Multifunctional Protein 1 Isoform A (SCYE1) ELISA Kit for Human
Human Aminoacyl tRNA Synthase Complex-Interacting Multifunctional Protein 1 (SCYE1) ELISA Kit
Gene Names
AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
Reactivity
Human
Synonyms
Aminoacyl tRNA Synthase Complex-Interacting Multifunctional Protein 1 (SCYE1); N/A; Human Aminoacyl tRNA Synthase Complex-Interacting Multifunctional Protein 1 (SCYE1) ELISA Kit; SCYE1; EMAP2; EMAPII; p43; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide; multisynthetase complex auxiliary co; SCYE1 elisa kit
Product Overview
Reactivity
Human
Specificity
Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCYE1. No significant cross-reactivity or interference between Human SCYE1 and analogues was observed.
Sequence Length
312
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) Standard Curve.)
Related Product Information for SCYE1 elisa kit
Description: This Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit employs a two-site sandwich ELISA to quantitate SCYE1 in samples. An antibody specific for SCYE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCYE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCYE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCYE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: AIMP1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.EMAPII is identical to the p43 subunit invariably associated with 9 aminoacyl-tRNA synthetase complexes. They stated that the C-terminal domain of p43 is an RNA-binding domain. EMAPII may be a bifunctional protein, binding RNA as part of the tRNA synthetase complexes and, after proteolytic cleavage in tumor cells, stimulating inflammatory responses.
Background: AIMP1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.EMAPII is identical to the p43 subunit invariably associated with 9 aminoacyl-tRNA synthetase complexes. They stated that the C-terminal domain of p43 is an RNA-binding domain. EMAPII may be a bifunctional protein, binding RNA as part of the tRNA synthetase complexes and, after proteolytic cleavage in tumor cells, stimulating inflammatory responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform a
NCBI Official Synonym Full Names
aminoacyl tRNA synthetase complex interacting multifunctional protein 1
NCBI Official Symbol
AIMP1
NCBI Official Synonym Symbols
p43; HLD3; EMAP2; SCYE1; EMAPII
NCBI Protein Information
aminoacyl tRNA synthase complex-interacting multifunctional protein 1
UniProt Protein Name
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
UniProt Gene Name
AIMP1
UniProt Synonym Gene Names
EMAP2; SCYE1; EMAP-2; EMAP-II
UniProt Entry Name
AIMP1_HUMAN
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Additional Details
Product Notes
The Human SCYE1 aimp1 (Catalog #AAA330940) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330940 ELISA Kit recognizes Human SCYE1. It is sometimes possible for the material contained within the vial of "Aminoacyl tRNA Synthase Complex-Interacting Multifunctional Protein 1 (SCYE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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