Standard Curve (Sample)
(Fig.1. Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) Standard Curve.)
A Disintegrin And Metalloproteinase With Thrombospondin Motifs 6 Preproprotein (ADAMTS6) ELISA Kit for Human
Human A Disintegrin And Metalloproteinase With Thrombospondin Motifs 6 (ADAMTS6) ELISA Kit
Gene Names
ADAMTS6; ADAM-TS6; ADAMTS-6; ADAM-TS 6
Reactivity
Human
Synonyms
A Disintegrin And Metalloproteinase With Thrombospondin Motifs 6 (ADAMTS6); N/A; Human A Disintegrin And Metalloproteinase With Thrombospondin Motifs 6 (ADAMTS6) ELISA Kit; ADAMTS6; ADAM-TS6; a disintegrin and metalloproteinase with thrombospondin motifs 6; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 6; ADAMTS6 elisa kit
Product Overview
Reactivity
Human
Specificity
Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTS6. No significant cross-reactivity or interference between Human ADAMTS6 and analogues was observed.
Sequence Length
1117
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) Standard Curve.)
Related Product Information for ADAMTS6 elisa kit
Description: This Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS6 in samples. An antibody specific for ADAMTS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The predicted 860-amino acid ADAMTS6 protein has a domain structure characteristic of ADAMTSs. Beginning at the N terminus, it has a preproregion containing a potential furin cleavage site and a putative cysteine-switch, a catalytic domain containing a typical reprolysin-type zinc-binding signature and a 'met turn,' a disintegrin-like domain, a conserved thrombospondin module, a cysteine-rich domain, a spacer domain, and a second, less conserved thrombospondin module. The mature ADAMTS6 protein has 2 potential N-linked glycosylation sites. Northern blot analysis of several human tissues detected an approximately 8.5-kb ADAMTS6 transcript that was expressed at low levels in placenta and at barely detectable levels in a number of other tissues. Adamts6 was not detectably expressed during mouse embryo development.
Background: The predicted 860-amino acid ADAMTS6 protein has a domain structure characteristic of ADAMTSs. Beginning at the N terminus, it has a preproregion containing a potential furin cleavage site and a putative cysteine-switch, a catalytic domain containing a typical reprolysin-type zinc-binding signature and a 'met turn,' a disintegrin-like domain, a conserved thrombospondin module, a cysteine-rich domain, a spacer domain, and a second, less conserved thrombospondin module. The mature ADAMTS6 protein has 2 potential N-linked glycosylation sites. Northern blot analysis of several human tissues detected an approximately 8.5-kb ADAMTS6 transcript that was expressed at low levels in placenta and at barely detectable levels in a number of other tissues. Adamts6 was not detectably expressed during mouse embryo development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 6 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 6
NCBI Official Symbol
ADAMTS6
NCBI Official Synonym Symbols
ADAM-TS6; ADAMTS-6; ADAM-TS 6
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 6
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 6
UniProt Gene Name
ADAMTS6
UniProt Synonym Gene Names
ADAM-TS 6; ADAM-TS6; ADAMTS-6
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Product Notes
The Human ADAMTS6 adamts6 (Catalog #AAA331111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331111 ELISA Kit recognizes Human ADAMTS6. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloproteinase With Thrombospondin Motifs 6 (ADAMTS6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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