Human 14 3 3 protein epsilon (YWHAE) ELISA Kit | YWHAE elisa kit

Human 14 3 3 protein epsilon (YWHAE) ELISA Kit

Cat. Num. AAA244396

• Gene Names: YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• Reactivity: Human
• Synonyms: 14 3 3 protein epsilon (YWHAE); N/A; Human 14 3 3 protein epsilon (YWHAE) ELISA Kit; YWHAE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of YWHAE. No significant cross-reactivity or interference between YWHAE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAE and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for YWHAE elisa kit

Principle of the Assay: YWHAE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YWHAE antibody and an YWHAE-HRP conjugate. The assay sample and buffer are incubated together with YWHAE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAE concentration since YWHAE from samples and YWHAE-HRP conjugate compete for the anti-YWHAE antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAE from the sample, fewer sites are left to bind YWHAE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAE concentration in each sample is interpolated from this standard curve.

Intended Uses: This YWHAE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YWHAE. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for YWHAE elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761.4
• Molecular Weight: 26,504 Da
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• UniProt Protein Name: 14-3-3 protein epsilon
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_HUMAN