FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)
anti-Human CD354 Antibody | anti-CD354 antibody
Anti-Hu CD354 Purified
Gene Names
TREM1; CD354; TREM-1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD354; N/A; Anti-Hu CD354 Purified; triggering receptor expressed on myeloid cells 1; TREM-1; anti-CD354 antibody
Reactivity
Human
Isotype
Mouse IgG1
Clone Number
6B1
Specificity
The mouse monoclonal antibody 6B1 (also known as 6B1.1G12) recognizes an extracellular epitope of human CD354 (TREM-1), a transmembrane glycoprotein, serving as an important innate immunity receptor.
Form/Format
Purified
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD354 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
recombinant human CD354 fused with IgG
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD354 (6B1) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD354 (6B1) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)
Related Product Information for anti-CD354 antibody
CD354 (TREM-1), a cell surface glycoprotein expressed mainly on monocytes, macrophages, and neutrophils, but also on e.g. endothelial cells or bronchial epithelium, plays an important role in innate immune responses. Upon triggering by its ligands (mainly bacterial and fungal components, but also some viruses, such as Marburg or Ebola virus), it initiates signaling cascades leading to release of pro-inflammatory cytokines and chemokines. CD354 is strongly present in infectious inflammatory lesions (e.g. in folliculitis or impetigo), but not so much in non-infectious inflammatory lesions, (e.g. vasculitis or psoriasis). However, under certain conditions neutrophils can upregulate CD354 also under non-infectious inflammatory conditions, e.g. in rheumatoid arthritis. Soluble CD354 (sTREM-1) is an important biomarker for identification of septic patients, as well as for prediction of their survival.
References
*Kuemmel A, Alflen A, Schmidt LH, Sebastian M, Wiewrodt R, Schulze AB, Buhl R, Radsak M: Soluble triggering receptor expressed on myeloid cells 1 in lung cancer. Sci Rep. 2018 Jul 17;8(1):10766., URL: https://pubmed.ncbi.nlm.nih.gov/30018308/,*Alflen A, Stadler N, Lopez PA, Teschner D, Theobald M, Hebeta G, Radsak MP: Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep. 2018 Apr 3;8(1):5558., URL: https://pubmed.ncbi.nlm.nih.gov/29615799/,*Prüfer S, Weber M, Sasca D, Teschner D, Wölfel C, Stein P, Stassen M, Schild H, Radsak MP: Distinct signaling cascades of TREM-1, TLR and NLR in neutrophils and monocytic cells. J Innate Immun. 2014;6(3):339-52., URL: https://pubmed.ncbi.nlm.nih.gov/24281714/,*Hasibeder A, Stein P, Brandwijk R, Schild H, Radsak MP: Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay. Sci Rep. 2015 Oct 20;5:15381., URL: https://pubmed.ncbi.nlm.nih.gov/26480887/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,387 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 1 isoform 2
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 1
NCBI Official Symbol
TREM1
NCBI Official Synonym Symbols
CD354; TREM-1
NCBI Protein Information
triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1
UniProt Protein Name
Triggering receptor expressed on myeloid cells 1
UniProt Gene Name
TREM1
UniProt Synonym Gene Names
TREM-1
UniProt Entry Name
TREM1_HUMAN
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Product Notes
The CD354 trem1 (Catalog #AAA129257) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD354 Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD354 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD354 trem1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD354, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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