Application Data
Igg (H&L), F(Ab)'2 Fragment (IGG) Secondary Antibody – Goat
Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment, TRITC Conjugate
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Antibody was affinity purified using solid phase Mouse IgG. F(ab)'2 fragment was prepared by pepsin digestion. Purity: Affinity purified antibody is >= 90% based on SDS-PAGE. Small amounts of intact IgG may be present.
Synonyms
IgG (H&L), F(ab)'2 Fragment; N/A; Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment, TRITC Conjugate; Goat anti-mouse IgG (H&L), F(ab)'2 fragment; IgG (H&L), F(ab)'2 Fragment secondary antibody
Product Overview
Host
Goat
Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on Mouse IgG
Light chains on all Mouse immunoglobulins
Light chains on all Mouse immunoglobulins
Purity/Purification
Antibody was affinity purified using solid phase Mouse IgG. F(ab)'2 fragment was prepared by pepsin digestion. Purity: Affinity purified antibody is >= 90% based on SDS-PAGE. Small amounts of intact IgG may be present.
Form/Format
Lyophilized
Concentration
0.5 mg of antibody at l.00 mg/ml (E 1% at 280 nm = 13.0) (varies by lot)
Applicable Applications for IgG (H&L), F(ab)'2 Fragment secondary antibody
FLISA, IF (Immmunofluorescence), IM, IHC (Immunohistochemsitry), FACS (Flow Cytometry)
Immunogen
Purified Mouse IgG, whole molecule
Fluorophore
Tetramethylrhodamine-5-isothiocyanate (TRITC) Ex = 550 nm; Em = 570 nm
Fluor/Protein Ratio
2.3 Moles TRITC per Mole Antibody
Crossreactivity
Based on IEP, no reactivity is observed to: non-immunoglobulin Mouse serum proteins
IgG from Bovine, Goat, Human, Rabbit, or Rat
IgG from Bovine, Goat, Human, Rabbit, or Rat
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05 % (w/v) Sodium Azide
Reconstitution
Rehydrate with 0.55 ml of deionized water. Allow solution to stand at room temperature for at least 30 minutes to dissolve. Centrifuge to remove any remaining particulates. Prepare fresh working dilution daily.
Preparation and Storage
Store freeze dried powder at 2-8 °C. Shelf Life: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.
Application Data
Application Data
Product Categories/Family for IgG (H&L), F(ab)'2 Fragment secondary antibody
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Additional Details
Product Notes
The IgG (H&L), F(ab)'2 Fragment (Catalog #AAA686078) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG (H&L), F(ab)'2 Fragment can be used in a range of immunoassay formats including, but not limited to, FLISA, IF (Immmunofluorescence), IM, IHC (Immunohistochemsitry), FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IgG (H&L), F(ab)'2 Fragment for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), F(ab)'2 Fragment, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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