Standard Curve (Sample)
Mouse Fas ligand (TNF superfamily, member 6) ELISA Kit | FASLG elisa kit
Mouse Factor-related Apoptosis ligand, FASL ELISA Kit
Reactivity
Mouse
Synonyms
Fas ligand (TNF superfamily, member 6); N/A; Mouse Factor-related Apoptosis ligand, FASL ELISA Kit; Mouse Factor-related Apoptosis ligand; FASL ELISA Kit; APT1LG1; CD178; CD95L; FASL; TNFSF6; CD95 ligand; apoptosis (APO-1) antigen ligand 1; fas ligand; tumor necrosis factor (ligand) superfamily; member 6; Fas ligand (TNF superfamily; member 6); FASLG elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse FASLG. No significant cross-reactivity or interference between Mouse FASLG and analogues was observed.
Sequence Length
279
Samples
Serum, plasma, tissue homogenates
Detection Range
7.8 pg/ml-500 pg/ml
Sensitivity
1.95 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Apoptosis/Autophagy
Protein Biological Process 3
Apoptosis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FASLG elisa kit
Introduction: FasL is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. The binding of FasLwith its receptor induces apoptosis. FasL-receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Soluble FasL is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external serine matrix metalloproteinase MMP-7. Soluble FasL is less active than their membrane-bound counterparts and do not induce receptor trimerization and DISC formation.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FASL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FASL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FASL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FASL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FASL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FASL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Product Notes
The Mouse FASLG (Catalog #AAA80324) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80324 ELISA Kit recognizes Mouse FASLG. It is sometimes possible for the material contained within the vial of "Fas ligand (TNF superfamily, member 6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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