Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ELISA Kit | ETFDH elisa kit

Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ELISA Kit

Cat. Num. AAA100557

• Gene Names: ETFDH; MADD; ETFQO
• Reactivity: Human
• Synonyms: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial; N/A; Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ELISA Kit; ETFDH (Electron transfer flavoprotein-ubiquinone oxidoreductase; mitochondrial)/ETF-QO/ETF-ubiquinone oxidoreductase/ETF dehydrogenase; ETFDH elisa kit

• Reactivity: Human
• Sequence Length: 617
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml

• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Standard Curve (Sample)

Related Product Information for ETFDH elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ETFDH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ETFDH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ETFDH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ETFDHcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119703746
• NCBI GeneID: 2110
• NCBI Accession #: NP_004444.2
• NCBI GenBank Nucleotide #: NM_004453.3
• Molecular Weight: 62,825 Da
• NCBI Official Full Name: electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: electron transfer flavoprotein dehydrogenase
• NCBI Official Symbol: ETFDH
• NCBI Official Synonym Symbols: MADD; ETFQO
• NCBI Protein Information: electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
• UniProt Protein Name: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
• UniProt Gene Name: ETFDH
• UniProt Synonym Gene Names: ETF-QO; ETF-ubiquinone oxidoreductase; ETF dehydrogenase
• UniProt Entry Name: ETFD_HUMAN