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ELISA Kits

AAA Biotech (AAA Bio or AAABio) provides ready-to-use Enzyme-Linked Immunosorbent Assay (ELISA) kits. These kits are used to detect and quantify a variety of antigens, proteins, peptides, hormones, cytokines, and other biomarkers. Our kits can be used with a variety of biological samples, such as serum, plasma, tissue homogenates, cell culture lysates, and supernatants.


Our validated kits are trusted by researchers worldwide for their precision, reliability, and ease of use. Researchers take advantage of our high-quality kits for studying biochemical pathways, disease monitoring and progressions, pathogen detection, pharmacological testing, biomarker discovery, etc.

However, if you can’t find the target or species you need for your research, don’t worry; we provide Custom Assay development services as well. So, all you need to do is submit your request, and we will determine if we can design an ELISA testing kit as per your specifications.


Viewing 1250-1300 of 115281 product results


Mouse AOAH ELISA Kit – Cat# AAA244410 – Standard Curve (Sample) Standard Curve (Sample)

Acyloxyacyl hydrolase (AOAH), ELISA Kit (Cat# AAA244410)

No reviews yet
Full Name
Mouse Acyloxyacyl hydrolase (AOAH) ELISA Kit
Reactivity
Mouse
Pricing
Guinea Pig HS ELISA Kit – Cat# AAA244418 – Standard Curve (Sample) Standard Curve (Sample)

heparan sulfate, HS, ELISA Kit (Cat# AAA244418)

No reviews yet
Full Name
Guinea pig heparan sulfate, HS ELISA Kit
Reactivity
Guinea Pig
Pricing
Chicken RPS18 ELISA Kit – Cat# AAA244471 – Standard Curve (Sample) Standard Curve (Sample)

40S ribosomal protein S18 (RPS18), ELISA Kit (Cat# AAA244471)

No reviews yet
Full Name
Chicken 40S ribosomal protein S18 (RPS18) ELISA Kit
Gene Names
RPS18; KE3; S18; HKE3; KE-3; D6S218E
Reactivity
Chicken
Pricing
Human EPB41L2 ELISA Kit – Cat# AAA244652 – Standard Curve (Sample) Standard Curve (Sample)

Band 4.1 like protein 2 (EPB41L2), ELISA Kit (Cat# AAA244652)

No reviews yet
Full Name
Human Band 4.1 like protein 2 (EPB41L2) ELISA Kit
Gene Names
EPB41L2; 4.1G; 4.1-G
Reactivity
Human
Pricing

Collagen, type V, alpha 1 (COL5A1), ELISA Kit (Cat# AAA244668)

No reviews yet
Full Name
Bovine Collagen, type V, alpha 1 (COL5A1) ELISA Kit
Reactivity
Bovine
Pricing
Chicken PLA2G5 ELISA Kit – Cat# AAA244679 – Standard Curve (Sample) Standard Curve (Sample)

Calcium-dependent phospholipase A2 (PLA2G5), ELISA Kit (Cat# AAA244679)

No reviews yet
Full Name
Chicken Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit
Gene Names
PLA2G5; FRFB; GV-PLA2; PLA2-10; hVPLA(2)
Reactivity
Chicken
Pricing
Human Anti ELISA Kit – Cat# AAA244700 – Standard Curve (Sample) Standard Curve (Sample)

Anti tissue transglutaminase IgG, IgG tTG, ELISA Kit (Cat# AAA244700)

No reviews yet
Full Name
Human Anti tissue transglutaminase IgG, IgG tTG ELISA Kit
Reactivity
Human
Pricing
Human CLGN ELISA Kit – Cat# AAA245714 – Standard Curve (Sample) Standard Curve (Sample)

Calmegin (CLGN), ELISA Kit (Cat# AAA245714)

No reviews yet
Full Name
Human Calmegin (CLGN) ELISA Kit
Reactivity
Human
Pricing
Rat SCD ELISA Kit – Cat# AAA245715 – Standard Curve (Sample) Standard Curve (Sample)

Acyl-CoA desaturase (SCD), ELISA Kit (Cat# AAA245715)

No reviews yet
Full Name
Rat Acyl-CoA desaturase (SCD) ELISA Kit
Gene Names
SCD; SCD1; FADS5; SCDOS; MSTP008
Reactivity
Rat
Pricing
Mouse CHDH ELISA Kit – Cat# AAA245724 – Standard Curve (Sample) Standard Curve (Sample)

Choline dehydrogenase, mitochondrial (CHDH), ELISA Kit (Cat# AAA245724)

No reviews yet
Full Name
Mouse Choline dehydrogenase, mitochondrial (CHDH) ELISA Kit
Reactivity
Mouse
Pricing
Human CFC1B ELISA Kit – Cat# AAA245730 – Standard Curve (Sample) Standard Curve (Sample)

Cryptic family protein 1B (CFC1B), ELISA Kit (Cat# AAA245730)

No reviews yet
Full Name
Human Cryptic family protein 1B (CFC1B) ELISA Kit
Reactivity
Human
Pricing
Rat BCAT2 ELISA Kit – Cat# AAA245731 – Standard Curve (Sample) Standard Curve (Sample)

Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2), ELISA Kit (Cat# AAA245731)

No reviews yet
Full Name
Rat Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) ELISA Kit
Gene Names
Bcat2; BCT2; Bcatm; mBcat
Reactivity
Rat
Pricing
Human CDC5L ELISA Kit – Cat# AAA245740 – Standard Curve (Sample) Standard Curve (Sample)

Cell division cycle 5 like protein (CDC5L), ELISA Kit (Cat# AAA245740)

No reviews yet
Full Name
Human Cell division cycle 5 like protein (CDC5L) ELISA Kit
Gene Names
CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
Reactivity
Human
Pricing
Human BCAT1 ELISA Kit – Cat# AAA245743 – Standard Curve (Sample) Standard Curve (Sample)

Branched chain amino acid aminotransferase, cytosolic (BCAT1), ELISA Kit (Cat# AAA245743)

No reviews yet
Full Name
Human Branched chain amino acid aminotransferase, cytosolic (BCAT1) ELISA Kit
Reactivity
Human
Pricing
Human T ELISA Kit – Cat# AAA245765 – Standard Curve (Sample) Standard Curve (Sample)

Brachyury protein (T), ELISA Kit (Cat# AAA245765)

No reviews yet
Full Name
Human Brachyury protein (T) ELISA Kit
Gene Names
T; TFT; SAVA
Reactivity
Human
Pricing
Mouse C8A ELISA Kit – Cat# AAA245781 – Standard Curve (Sample) Standard Curve (Sample)

Complement component C8 alpha chain (C8A), ELISA Kit (Cat# AAA245781)

No reviews yet
Full Name
Mouse Complement component C8 alpha chain (C8A) ELISA Kit
Reactivity
Mouse
Pricing
Human CRIP1 ELISA Kit – Cat# AAA245795 – Standard Curve (Sample) Standard Curve (Sample)

Cysteine rich protein 1 (CRIP1), ELISA Kit (Cat# AAA245795)

No reviews yet
Full Name
Human Cysteine rich protein 1 (CRIP1) ELISA Kit
Reactivity
Human
Pricing
Mouse CSN2 ELISA Kit – Cat# AAA245811 – Standard Curve (Sample) Standard Curve (Sample)

Beta-casein (CSN2), ELISA Kit (Cat# AAA245811)

No reviews yet
Full Name
Mouse Beta-casein (CSN2) ELISA Kit
Gene Names
CSN2; CASB
Reactivity
Mouse
Pricing
Mouse CSPG4 ELISA Kit – Cat# AAA245818 – Standard Curve (Sample) Standard Curve (Sample)

Chondroitin sulfate proteoglycan 4 (CSPG4), ELISA Kit (Cat# AAA245818)

No reviews yet
Full Name
Mouse Chondroitin sulfate proteoglycan 4 (CSPG4) ELISA Kit
Gene Names
CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Reactivity
Mouse
Pricing
Rat GJC1 ELISA Kit – Cat# AAA245822 – Standard Curve (Sample) Standard Curve (Sample)

Gap junction gamma-1 protein (GJC1), ELISA Kit (Cat# AAA245822)

No reviews yet
Full Name
Rat Gap junction gamma-1 protein (GJC1) ELISA Kit
Gene Names
GJC1; CX45; GJA7
Reactivity
Rat
Pricing
Human LAP3 ELISA Kit – Cat# AAA245845 – Standard Curve (Sample) Standard Curve (Sample)

leucine aminopeptidase 3 (LAP3), ELISA Kit (Cat# AAA245845)

No reviews yet
Full Name
Human leucine aminopeptidase 3 (LAP3) ELISA Kit
Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Human
Pricing
Human CYP46A1 ELISA Kit – Cat# AAA245851 – Standard Curve (Sample) Standard Curve (Sample)

Cholesterol 24 hydroxylase (CYP46A1), ELISA Kit (Cat# AAA245851)

No reviews yet
Full Name
Human Cholesterol 24 hydroxylase (CYP46A1) ELISA Kit
Gene Names
CYP46A1; CP46; CYP46
Reactivity
Human
Pricing
Human NAPB ELISA Kit – Cat# AAA245852 – Standard Curve (Sample) Standard Curve (Sample)

beta soluble NSF attachment protein (NAPB), ELISA Kit (Cat# AAA245852)

No reviews yet
Full Name
Human beta soluble NSF attachment protein (NAPB) ELISA Kit
Gene Names
NAPB; SNAPB; SNAP-BETA
Reactivity
Human
Pricing
Sheep UCP2 ELISA Kit – Cat# AAA245857 – Standard Curve (Sample) Standard Curve (Sample)

Mitochondrial uncoupling protein 2 (UCP2), ELISA Kit (Cat# AAA245857)

No reviews yet
Full Name
Sheep Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit
Gene Names
UCP2; UCPH; BMIQ4; SLC25A8
Reactivity
Sheep
Pricing
Human COX6C ELISA Kit – Cat# AAA245858 – Standard Curve (Sample) Standard Curve (Sample)

Cytochrome c oxidase subunit 6C (COX6C), ELISA Kit (Cat# AAA245858)

No reviews yet
Full Name
Human Cytochrome c oxidase subunit 6C (COX6C) ELISA Kit
Reactivity
Human
Pricing
Rat CLDN11 ELISA Kit – Cat# AAA245859 – Standard Curve (Sample) Standard Curve (Sample)

Claudin-11 (CLDN11), ELISA Kit (Cat# AAA245859)

No reviews yet
Full Name
Rat Claudin-11 (CLDN11) ELISA Kit
Gene Names
CLDN11; OSP; OTM
Reactivity
Rat
Pricing
Mouse CLEC1B ELISA Kit – Cat# AAA245869 – Standard Curve (Sample) Standard Curve (Sample)

C-type lectin domain family 1 member B (CLEC1B), ELISA Kit (Cat# AAA245869)

No reviews yet
Full Name
Mouse C-type lectin domain family 1 member B (CLEC1B) ELISA Kit
Gene Names
CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
Reactivity
Mouse
Pricing
Human CCDC40 ELISA Kit – Cat# AAA245872 – Standard Curve (Sample) Standard Curve (Sample)

Coiled coil domain containing protein 40 (CCDC40), ELISA Kit (Cat# AAA245872)

No reviews yet
Full Name
Human Coiled coil domain containing protein 40 (CCDC40) ELISA Kit
Gene Names
CCDC40; CILD15; FAP172
Reactivity
Human
Pricing
Human CXCR6 ELISA Kit – Cat# AAA245964 – Standard Curve (Sample) Standard Curve (Sample)

C X C chemokine receptor type 6 (CXCR6), ELISA Kit (Cat# AAA245964)

No reviews yet
Full Name
Human C X C chemokine receptor type 6 (CXCR6) ELISA Kit
Gene Names
CXCR6; BONZO; CD186; STRL33; TYMSTR
Reactivity
Human
Pricing
Bovine AJAP1 ELISA Kit – Cat# AAA245965 – Standard Curve (Sample) Standard Curve (Sample)

Adherens junction-associated protein 1 (AJAP1), ELISA Kit (Cat# AAA245965)

No reviews yet
Full Name
Bovine Adherens junction-associated protein 1 (AJAP1) ELISA Kit
Gene Names
AJAP1; MOT8; SHREW1; SHREW-1
Reactivity
Bovine
Pricing
Human CEND1 ELISA Kit – Cat# AAA245976 – Standard Curve (Sample) Standard Curve (Sample)

Cell cycle exit and neuronal differentiation protein 1 (CEND1), ELISA Kit (Cat# AAA245976)

No reviews yet
Full Name
Human Cell cycle exit and neuronal differentiation protein 1 (CEND1) ELISA Kit
Gene Names
CEND1; BM88
Reactivity
Human
Pricing
Mouse ORAI1 ELISA Kit – Cat# AAA246001 – Standard Curve (Sample) Standard Curve (Sample)

Calcium release-activated calcium channel protein 1 (ORAI1), ELISA Kit (Cat# AAA246001)

No reviews yet
Full Name
Mouse Calcium release-activated calcium channel protein 1 (ORAI1) ELISA Kit
Gene Names
ORAI1; IMD9; TAM2; ORAT1; CRACM1; TMEM142A
Reactivity
Mouse
Pricing
Human SLC26A7 ELISA Kit – Cat# AAA246009 – Standard Curve (Sample) Standard Curve (Sample)

Anion exchange transporter (SLC26A7), ELISA Kit (Cat# AAA246009)

No reviews yet
Full Name
Human Anion exchange transporter (SLC26A7) ELISA Kit
Gene Names
SLC26A7; SUT2
Reactivity
Human
Pricing
Mouse CCDC3 ELISA Kit – Cat# AAA246014 – Standard Curve (Sample) Standard Curve (Sample)

Coiled-coil domain-containing protein 3 (CCDC3), ELISA Kit (Cat# AAA246014)

No reviews yet
Full Name
Mouse Coiled-coil domain-containing protein 3 (CCDC3) ELISA Kit
Reactivity
Mouse
Pricing
Human Ischemia ELISA Kit – Cat# AAA246018 – Standard Curve (Sample) Standard Curve (Sample)

Ischemia Modified Albumin, ELISA Kit (Cat# AAA246018)

No reviews yet
Full Name
Human Ischemia Modified Albumin ELISA Kit
Reactivity
Human
Pricing
Human ADH1B ELISA Kit – Cat# AAA246026 – Standard Curve (Sample) Standard Curve (Sample)

Alcohol dehydrogenase 1B (ADH1B), ELISA Kit (Cat# AAA246026)

No reviews yet
Full Name
Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit
Reactivity
Human
Pricing
Human FSHB ELISA Kit – Cat# AAA246032 – Standard Curve (Sample) Standard Curve (Sample)

Follitropin subunit beta (FSHB), ELISA Kit (Cat# AAA246032)

No reviews yet
Full Name
Human Follitropin subunit beta (FSHB) ELISA Kit
Reactivity
Human
Pricing
Human AGMAT ELISA Kit – Cat# AAA246035 – Standard Curve (Sample) Standard Curve (Sample)

Agmatine Ureohydrolase/Agmatinase (AGMAT), ELISA Kit (Cat# AAA246035)

No reviews yet
Full Name
Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit
Reactivity
Human
Pricing
Human CRX ELISA Kit – Cat# AAA246039 – Standard Curve (Sample) Standard Curve (Sample)

Cone rod homeobox protein (CRX), ELISA Kit (Cat# AAA246039)

No reviews yet
Full Name
Human Cone rod homeobox protein (CRX) ELISA Kit
Gene Names
CRX; CRD; LCA7; OTX3; CORD2
Reactivity
Human
Pricing
Human SGCB ELISA Kit – Cat# AAA246040 – Standard Curve (Sample) Standard Curve (Sample)

beta sarcoglycan (SGCB), ELISA Kit (Cat# AAA246040)

No reviews yet
Full Name
Human beta sarcoglycan (SGCB) ELISA Kit
Gene Names
SGCB; A3b; SGC; LGMD2E
Reactivity
Human
Pricing
Rat AMBN ELISA Kit – Cat# AAA246041 – Standard Curve (Sample) Standard Curve (Sample)

Ameloblastin (AMBN), ELISA Kit (Cat# AAA246041)

No reviews yet
Full Name
Rat Ameloblastin (AMBN) ELISA Kit
Reactivity
Rat
Pricing
Human ANO2 ELISA Kit – Cat# AAA245635 – Standard Curve (Sample) Standard Curve (Sample)

Anoctamin 2 (ANO2), ELISA Kit (Cat# AAA245635)

No reviews yet
Full Name
Human Anoctamin 2 (ANO2) ELISA Kit
Gene Names
ANO2; C12orf3; TMEM16B
Reactivity
Human
Pricing
Human BCAT2 ELISA Kit – Cat# AAA245672 – Standard Curve (Sample) Standard Curve (Sample)

Branched chain amino acid aminotransferase, mitochondrial (BCAT2), ELISA Kit (Cat# AAA245672)

No reviews yet
Full Name
Human Branched chain amino acid aminotransferase, mitochondrial (BCAT2) ELISA Kit
Gene Names
BCAT2; BCAM; BCT2; PP18; BCATM
Reactivity
Human
Pricing
Bovine CYB5R3 ELISA Kit – Cat# AAA245675 – Standard Curve (Sample) Standard Curve (Sample)

NADH-cytochrome b5 reductase 3 (CYB5R3), ELISA Kit (Cat# AAA245675)

No reviews yet
Full Name
Bovine NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit
Gene Names
CYB5R3; B5R; DIA1
Reactivity
Bovine
Pricing
Human ACP1 ELISA Kit – Cat# AAA245681 – Standard Curve (Sample) Standard Curve (Sample)

Low molecular weight phosphotyrosine protein phosphatase (ACP1), ELISA Kit (Cat# AAA245681)

No reviews yet
Full Name
Human Low molecular weight phosphotyrosine protein phosphatase (ACP1) ELISA Kit
Gene Names
ACP1; HAAP
Reactivity
Human
Pricing
Mouse ADCY2 ELISA Kit – Cat# AAA245694 – Standard Curve (Sample) Standard Curve (Sample)

Adenylate cyclase type 2 (ADCY2), ELISA Kit (Cat# AAA245694)

No reviews yet
Full Name
Mouse Adenylate cyclase type 2 (ADCY2) ELISA Kit
Gene Names
ADCY2; AC2; HBAC2
Reactivity
Mouse
Pricing
Rat C4orf35 ELISA Kit – Cat# AAA245705 – Standard Curve (Sample) Standard Curve (Sample)

Calcium-binding and spermatid-specific protein 1 (C4orf35), ELISA Kit (Cat# AAA245705)

No reviews yet
Full Name
Rat Calcium-binding and spermatid-specific protein 1 (C4orf35) ELISA Kit
Gene Names
CABS1; CLPH; C4orf35; NYD-SP26
Reactivity
Rat
Pricing
Rat PRKG1 ELISA Kit – Cat# AAA245709 – Standard Curve (Sample) Standard Curve (Sample)

cGMP-dependent protein kinase 1 (PRKG1), ELISA Kit (Cat# AAA245709)

No reviews yet
Full Name
Rat cGMP-dependent protein kinase 1 (PRKG1) ELISA Kit
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Rat
Pricing
Rat CPN2 ELISA Kit – Cat# AAA245888 – Standard Curve (Sample) Standard Curve (Sample)

Carboxypeptidase N subunit 2 (CPN2), ELISA Kit (Cat# AAA245888)

No reviews yet
Full Name
Rat Carboxypeptidase N subunit 2 (CPN2) ELISA Kit
Gene Names
CPN2; ACBP
Reactivity
Rat
Pricing
Human RPL36A ELISA Kit – Cat# AAA245891 – Standard Curve (Sample) Standard Curve (Sample)

60S ribosomal protein L36a (RPL36A), ELISA Kit (Cat# AAA245891)

No reviews yet
Full Name
Human 60S ribosomal protein L36a (RPL36A) ELISA Kit
Gene Names
rpl36a; bbr; rl44
Reactivity
Human
Pricing

Our catalog contains ELISA assay kits that are reactive with samples coming from a very wide range of different species, including:


  • Human
  • Mouse
  • Rat
  • Bovine
  • Rabbit
  • Chicken
  • Porcine
  • Monkey
  • Goat
  • Sheep
  • Canine
  • Guinea Pig
  • And more…

We also have kits that are compatible with samples originating from less common species, including:


  • Horse/Equine
  • Fish
  • Cat
  • Hamster
  • Donkey
  • Duck
  • Pigeon
  • Camel
  • Goose
  • Plant
  • And more…

Check out a comprehensive catalog of ready-to-use assay kits and place your order for ELISA kits online now! Want to learn more about ELISA? Here is our complete guide to ELISA.


What are the Benefits of ELISA Kits?


  • High sensitivity and specificity: ELISA is capable of detecting even low levels of analytes with excellent accuracy.

  • Quantitative results: Provides precise antigen and antibody concentration results.

  • Scalable and high throughput: Ideal for analyzing large numbers of samples at the same time.

  • Easy to use: Intuitive and straightforward procedure.

  • Versatile applications: Ideal for use in immunology, cancer research, infectious disease, food safety, and more.


What is Included in an ELISA Kit?


Each ELISA kit from AAA Biotech typically contains the following components:


  • Pre-coated 96/48/24-well microplate

  • Standards and/or controls

  • Detection antibody

  • Enzyme conjugate

  • Substrate solution (e.g., TMB)

  • Stop solution

  • Sample diluent and wash buffer

  • Detailed protocol and instructions

Why Buy ELISA Kits from AAA Biotech?


  • Quick and Convenient Ordering: Simple online ordering process with responsive customer support.

  • Wide Selection of ELISA Kits: Extensive product range covering wide range of species, targets, and research areas.

  • Affordable Prices: High-quality kits at competitive prices to support all research budgets.

  • Over 10 Years Supporting the Research Community: A dedicated partner to researchers worldwide.

  • Shipping to 100+ Countries: Reliable international delivery with tracked shipments.

  • High-Quality, Validated Reagents: Designed for accuracy, reproducibility, and performance in research settings.

  • Trusted by Researchers Globally: Used in universities, biotech labs, and research institutes around the world.

  • Various Applications: Ideal for basic science, immunology, oncology, infectious disease study, and more.

When to Choose a Custom ELISA Kit, When to Buy a Ready-to-Use ELISA Kit?


Requirements for an ELISA kit can vary from project-to-project. Here are some strategies for making an informed decision about whether to go with a ready-to-use or a customized ELISA kit:


When to Choose a Ready-to-Use ELISA Kit:


  • Your target analyte is already available in the catalog

  • You need quick and reliable results without additional optimization

  • You are using “typical” sample types like serum, plasma, or cell culture supernatants

  • Your project requires cost-effective, validated, and widely used assays


When to Choose a Custom ELISA Kit:


  • Your analyte of interest is rare, novel, or not covered in existing kits

  • You are working with uncommon species or non-standard sample types

  • You need a specific assay format (competitive, sandwich, or indirect) tailored to your study

  • Your research demands unique sensitivity, specificity, or detection ranges

  • You want the assay optimized for your lab’s workflow and requirements


AAA Biotech supports both ready-to-use ELISA kits and Custom Assay Development Services, giving researchers the flexibility to select the best solution for their scientific goals.


ELISA Kit Protocol


The user manuals for our ELISA kits are conveniently accessible through the datasheets on our website or can be provided upon request. Each manual contains a clear explanation of the assay principle, a complete list of kit components, and a step-by-step protocol. These guides are designed to be straightforward, making them suitable for researchers who are new to ELISA techniques as well as experienced users who need a quick, reliable reference. In addition, Safety Data Sheets (SDS) for all products are available upon request to ensure safe handling.


What sets AAA Biotech apart is not just the range of assays we offer, but the consistency and reliability of our reagents. Every kit is carefully validated, ensuring that researchers can trust the results for both routine testing and advanced studies.


FAQ


1. When should I use a competitive ELISA assay?


Competitive ELISA is ideal when the target analyte is a smaller molecule (such as hormones or other short peptides or lipids/sugars) and there is unlikely to be sufficient surface or possible epitopes on the analyte/molecule to allow for two antibodies to be able to bind to it simultaneously (as would be required by an ELISA using the traditional “sandwich” design format).


2. What samples can ELISA kits be used for?


ELISA kits are compatible with a wide variety of biological samples, including serum, plasma, cell culture supernatants, urine, saliva, and tissue homogenates. Serum & plasma are routinely tested with the vast majority of kits in our catalog, but since many target analytes do not exist in these sample types, some will be further validated with various tissue homogenate or cell lysate samples. The samples ultimately tested by the manufacturing lab technician will largely depend on the target analyte’s typical location in an organism, or where its presence is of interest to the research community.


3. How long can I store my sample before using it for an ELISA assay?


Sample shelf-life ultimately depends on the intrinsic stability of the target analyte in the samples’ specific biological matrix, and also any other factors affecting it during storage (such as added preservatives and temperature). Generally:


  • Short-term (up to 48 hours): Store at 2–8°C
  • Long-term: Store at -20°C or -80°C for most biological samples

4. Where can I buy an ELISA test kit?


You can browse AAA Biotech’s comprehensive catalog and place your order directly through our website. For special requirements, you can also submit a request for a custom ELISA kit.


5. Why are ELISA tests costly?


ELISA kits involve high-quality reagents, validated antibodies, and precise controls to ensure accurate and reproducible results. Costs also reflect research and development, quality control, and the rigorous validation process required to support reliable scientific outcomes. While they may seem expensive, the investment ensures high sensitivity, specificity, and trustworthy data required by our users’ critical research.


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