ELISA Kits

At AAA Biotech, we provide Enzyme-Linked Immunosorbent Assay (ELISA) kits that provide accurate and reliable results. These kits can detect a wide range of antigens, hormones, proteins, peptides, etc. contained within a biological sample. Due to their high sensitivity and specificity, ELISA kits are used frequently in most laboratories involved in biological research.

Our catalog contains kits for detecting various target analytes in many different species, such as for humans, mice, rats, bovines, rabbits, chickens, monkeys, goats, sheep, and more. We also have ELISA testing kits that can react with less common species, such as horses, fish, cats, donkeys, and ducks. We have different types of ELISA kits for different formats – direct, indirect, sandwich, competitive, etc. Explore our range of ELISA assay kits to discover the one that best suits your needs, or contact our technical support team to help you in your search!

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G Protein Coupled Receptor 35 (GPR35), Assay Kit (Cat# AAA160066)

Full Name

G Protein Coupled Receptor 35 (GPR35) Magnetic Fluorescence Assay Kit

Reactivity

Human

Pricing

$1,030/96 Tests $2,000/2x96 Tests $2,925/3x96 Tests $3,795/4x96 Tests $4,145/5x96 Tests $7,565/10x96 Tests

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Glycine-N-Methyltransferase (GNMT), Assay Kit (Cat# AAA160103)

Full Name

Glycine-N-Methyltransferase (GNMT) Magnetic Fluorescence Assay Kit

Reactivity

Human

Pricing

$1,030/96 Tests $2,000/2x96 Tests $2,925/3x96 Tests $3,795/4x96 Tests $4,145/5x96 Tests $7,565/10x96 Tests

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NTRK2, ELISA Kit (Cat# AAA164257)

Full Name

Mouse NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit

Gene Names

NTRK2; TRKB; trk-B; GP145-TrkB

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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Sodium/glucose cotransporter 2, ELISA Kit (Cat# AAA56827)

Full Name

Human Sodium/glucose cotransporter 2 ELISA Kit

Gene Names

SLC5A2; SGLT2

Reactivity

Human

Pricing

$490/48-Strip-Wells $655/96-Strip-Wells $1,270/2x96-Strip-Wells $2,760/5x96-Strip-Wells $5,085/10x96-Strip-Wells

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Cadherin 26 (CDH26), Assay Kit (Cat# AAA161267)

Full Name

Cadherin 26 (CDH26) Magnetic Fluorescence Assay Kit

Reactivity

Human

Pricing

$1,120/96 Tests $2,175/2x96 Tests $3,180/3x96 Tests $4,125/4x96 Tests $4,505/5x96 Tests $8,190/10x96 Tests

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Non Metastatic Cells 1, Protein NM23A Expressed In (NME1), Assay Kit (Cat# AAA160694)

Full Name

Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Magnetic Fluorescence Assay Kit

Reactivity

Rat

Pricing

$1,110/96 Tests $2,155/2x96 Tests $3,150/3x96 Tests $4,090/4x96 Tests $4,450/5x96 Tests $8,085/10x96 Tests

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EphA2, ELISA Kit (Cat# AAA165151)

Full Name

Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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CREB Binding Protein (CREBBP), ELISA Kit (Cat# AAA148641)

Full Name

Rat CREB Binding Protein (CREBBP) ELISA Kit

Gene Names

Crebbp; CBP; RTS; RSTS

Reactivity

Rat

Pricing

$465/24-Strip-Wells $705/48-Strip-Wells $945/96-Strip-Wells $1,845/2x96-Strip-Wells $3,800/5x96-Strip-Wells $6,955/10x96-Strip-Wells

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Glucose Uptake, Assay Kit (Cat# AAA177831)

Full Name

Glucose Uptake Fluorometric Assay Kit

Pricing

$825/96 Tests $1,880/500 Assays $3,660/2x500 Assays $5,340/3x500 Assays $6,935/4x500 Assays $8,800/5x500 Assays

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MHCC/HLA-C, ELISA Kit (Cat# AAA164796)

Full Name

Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit

Reactivity

Human

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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IL-1alpha (Interleukin 1 Alpha), ELISA Kit (Cat# AAA178526)

Full Name

Uncoated Mouse IL-1alpha (Interleukin 1 Alpha) ELISA Kit

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $565/5x96 Tests

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Cofilin 1, Non Muscle (CFL1), ELISA Kit (Cat# AAA135887)

Full Name

Human Cofilin 1, Non Muscle (CFL1) ELISA Kit

Reactivity

Human

Pricing

$425/24-Strip-Wells $640/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,405/5x96-Strip-Wells $6,210/10x96-Strip-Wells

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Oxidized Low Density Lipoprotein (OxLDL), ELISA Kit (Cat# AAA136014)

Full Name

Rat Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

Reactivity

Rat

Standard Curve (Sample)

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Bromo Adjacent Homology Domain Containing Protein 2 (BAHD2), Assay Kit (Cat# AAA161420)

Full Name

Bromo Adjacent Homology Domain Containing Protein 2 (BAHD2) Magnetic Fluorescence Assay Kit

Reactivity

Human

Pricing

$1,120/96 Tests $2,175/2x96 Tests $3,180/3x96 Tests $4,125/4x96 Tests $4,505/5x96 Tests $8,190/10x96 Tests

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Anti-Mannose Binding Lectin Antibody (Anti-MBL), ELISA Kit (Cat# AAA129711)

Full Name

Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit

Reactivity

Human

Pricing

$570/24-Strip-Wells $880/48-Strip-Wells $1,195/96-Strip-Wells $2,330/2x96-Strip-Wells $4,815/5x96-Strip-Wells $8,745/10x96-Strip-Wells

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Platelet Activating Factor (PAF), ELISA Kit (Cat# AAA135737)

Full Name

Mouse Platelet Activating Factor (PAF) ELISA Kit

Reactivity

Mouse

Pricing

$435/24-Strip-Wells $635/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,280/5x96-Strip-Wells $5,885/10x96-Strip-Wells

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Bcl-2 associated X protein, Bax, ELISA Kit (Cat# AAA43197)

Full Name

Rat Bcl-2 associated X protein, Bax ELISA Kit

Reactivity

Rat

Pricing

$455/48-Strip-Wells $670/96-Strip-Wells $1,305/2x96-Strip-Wells $2,860/5x96-Strip-Wells $5,625/10x96-Strip-Wells

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Semaphorin 4D (SEMA4D), ELISA Kit (Cat# AAA43224)

Full Name

Human Semaphorin 4D (SEMA4D) ELISA Kit

Gene Names

SEMA4D; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G

Reactivity

Human

Pricing

$435/48-Strip-Wells $605/96-Strip-Wells $1,170/2x96-Strip-Wells $2,550/5x96-Strip-Wells $5,015/10x96-Strip-Wells

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PEA (Pseudomonas Exotoxin A), ELISA Kit (Cat# AAA249873)

Full Name

PEA (Pseudomonas Exotoxin A) ELISA Kit

Reactivity

General

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

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Thrombopoietin Receptor antibody, ELISA Kit (Cat# AAA163437)

Full Name

Mouse Thrombopoietin Receptor antibody ELISA Kit

Gene Names

THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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IL-5, ELISA Kit (Cat# AAA163914)

Full Name

Rat IL-5 (Interleukin 5) ELISA Kit

Reactivity

Rat

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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GLUT3, ELISA Kit (Cat# AAA164432)

Full Name

Human GLUT3 (Glucose Transporter 3) ELISA Kit

Gene Names

SLC2A3; GLUT3

Reactivity

Human

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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Matrix Metalloproteinase 19 (MMP19), Assay Kit (Cat# AAA157414)

Full Name

Matrix Metalloproteinase 19 (MMP19) Magnetic Fluorescence Assay Kit

Reactivity

Mouse

Pricing

$1,000/96 Tests $1,950/2x96 Tests $2,855/3x96 Tests $3,700/4x96 Tests $4,060/5x96 Tests $7,420/10x96 Tests

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Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1), Assay Kit (Cat# AAA160852)

Full Name

Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) Magnetic Fluorescence Assay Kit

Reactivity

Mouse

Pricing

$1,145/96 Tests $2,225/2x96 Tests $3,250/3x96 Tests $4,220/4x96 Tests $4,610/5x96 Tests $8,375/10x96 Tests

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DRD4, ELISA Kit (Cat# AAA164835)

Full Name

Mouse DRD4 (Dopamine Receptor D4) ELISA Kit

Gene Names

DRD4; D4DR

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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Histone, ELISA Kit (Cat# AAA93837)

Full Name

Goat Histone ELISA Kit

Gene Names

H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1

Reactivity

Goat

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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Optic Atrophy 1, Autosomal Dominant (OPA1), ELISA Kit (Cat# AAA150504)

Full Name

Human Optic Atrophy 1, Autosomal Dominant (OPA1) ELISA Kit

Reactivity

Human

Applications

ELISA

Pricing

$435/24-Strip-Wells $660/48-Strip-Wells $880/96-Strip-Wells $1,710/2x96-Strip-Wells $3,550/5x96-Strip-Wells $6,470/10x96-Strip-Wells

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PGC, ELISA Kit (Cat# AAA165230)

Full Name

Rat PGC (PepsinegenC) ELISA Kit

Gene Names

PGC; PEPC; PGII

Reactivity

Rat

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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CAV-1, ELISA Kit (Cat# AAA165247)

Full Name

Rat CAV-1 (Caveolin-1)ELISA Kit

Gene Names

Cav1; Cav; Cav-1

Reactivity

Rat

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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Application Data (This assay recognizes both recombinant and natural Human GDF15. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample) (This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

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GDF15, ELISA Kit (Cat# AAA258407)

Full Name

Human GDF15 ELISA Kit

Reactivity

Human

Pricing

$525/96 Tests $1,015/2x96 Tests $1,485/3x96 Tests $1,930/4x96 Tests $2,385/5x96 Tests

Standard Curve (Sample)

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Centromere Protein, CENP-B, ELISA Kit (Cat# AAA247084)

Full Name

Mouse Centromere Protein, CENP-B ELISA Kit

Reactivity

Mouse

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

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Left/Right Determination Factor 1 (LEFTY1), ELISA Kit (Cat# AAA137207)

Full Name

Mouse Left/Right Determination Factor 1 (LEFTY1) ELISA Kit

Reactivity

Mouse

Pricing

$440/24-Strip-Wells $670/48-Strip-Wells $900/96-Strip-Wells $1,750/2x96-Strip-Wells $3,635/5x96-Strip-Wells $6,650/10x96-Strip-Wells

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Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1), ELISA Kit (Cat# AAA135773)

Full Name

Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

Gene Names

MS4A4A; MS4A4; MS4A7; 4SPAN1; CD20L1; CD20-L1; HDCME31P

Reactivity

Human

Pricing

$425/24-Strip-Wells $640/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,405/5x96-Strip-Wells $6,210/10x96-Strip-Wells

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Armadillo Repeat-Containing Protein 6, ELISA Kit (Cat# AAA206223)

Full Name

Human Armadillo Repeat-Containing Protein 6 ELISA Kit

Reactivity

Human

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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Insulin Autoantibody, ELISA Kit (Cat# AAA90563)

Full Name

Goat Insulin Autoantibody ELISA Kit

Reactivity

Goat

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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GRO/MGSA, CLIA Kit (Cat# AAA318531)

Full Name

GRO/MGSA (Human) LumiAb ELISA Kit

Gene Names

CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a

Reactivity

Human

Pricing

$520/1 Kit $1,005/2x1 Kit $1,470/3x1 Kit $1,910/4x1 Kit $2,360/5x1 Kit

Standard Curve (Sample)

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Mucin 13, Cell Surface Associated (MUC13), Assay Kit (Cat# AAA161208)

Full Name

Mucin 13, Cell Surface Associated (MUC13) Magnetic Fluorescence Assay Kit

Reactivity

Human

Pricing

$1,120/96 Tests $2,175/2x96 Tests $3,180/3x96 Tests $4,125/4x96 Tests $4,505/5x96 Tests $8,190/10x96 Tests

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Uromodulin (UMOD), Assay Kit (Cat# AAA160137)

Full Name

Uromodulin (UMOD) Magnetic Fluorescence Assay Kit

Reactivity

Mouse

Pricing

$1,060/96 Tests $2,055/2x96 Tests $3,000/3x96 Tests $3,895/4x96 Tests $4,245/5x96 Tests $7,705/10x96 Tests

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Application Data

Application Data

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CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Fluorometric), Assay Kit (Cat# AAA44625)

Full Name

CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Fluorometric)

Pricing

$640/48 Assays $1,240/2x48 Assays $1,815/3x48 Assays $2,355/4x48 Assays $2,920/5x48 Assays

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Leptin Antibody, ELISA Kit (Cat# AAA244624)

Full Name

Guinea pig Leptin Antibody ELISA Kit

Reactivity

Guinea Pig

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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Wingless Type MMTV Integration Site Family, Member 3A (WNT3A), CLIA Kit (Cat# AAA130055)

Full Name

CLIA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)

Pricing

$430/24-Strip-Wells $780/48-Strip-Wells $1,060/96-Strip-Wells $2,055/2x96-Strip-Wells $4,145/5x96-Strip-Wells $7,440/10x96-Strip-Wells

Standard Curve (Sample)

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Reticulocalbin 3 (RCN3), ELISA Kit (Cat# AAA188273)

Full Name

Human Reticulocalbin 3 (RCN3) ELISA Kit

Reactivity

Human

Pricing

$310/24-Strip-Wells $405/48-Strip-Wells $505/96-Strip-Wells $985/2x96-Strip-Wells $2,130/5x96-Strip-Wells $4,020/10x96-Strip-Wells

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Cathepsin K, cath-K, ELISA Kit (Cat# AAA41539)

Full Name

Rat Cathepsin K, cath-K ELISA Kit

Gene Names

CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2

Reactivity

Rat

Pricing

$455/48-Strip-Wells $670/96-Strip-Wells $1,305/2x96-Strip-Wells $2,860/5x96-Strip-Wells $5,625/10x96-Strip-Wells

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Caseinolytic peptidase B protein homolog (CLPB), ELISA Kit (Cat# AAA246912)

Full Name

Human Caseinolytic peptidase B protein homolog (CLPB) ELISA Kit

Gene Names

CLPB; SKD3; HSP78

Reactivity

Human

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

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Collagen Type III Alpha 1 (COL3a1), ELISA Kit (Cat# AAA140881)

Full Name

Mouse Collagen Type III Alpha 1 (COL3a1) ELISA Kit

Gene Names

COL3A1; EDS4A

Reactivity

Mouse

Pricing

$440/24-Strip-Wells $670/48-Strip-Wells $900/96-Strip-Wells $1,750/2x96-Strip-Wells $3,635/5x96-Strip-Wells $6,650/10x96-Strip-Wells

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Des, ELISA Kit (Cat# AAA163458)

Full Name

Mouse Des (Desmin) ELISA Kit

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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CASP9, ELISA Kit (Cat# AAA163744)

Full Name

Mouse CASP9 (Caspase 9) ELISA Kit

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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PVRL3, ELISA Kit (Cat# AAA164137)

Full Name

Mouse PVRL3 (Poliovirus Receptor Related Protein 3) ELISA Kit

Gene Names

PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

Standard Curve (Sample)

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TGF-beta1, ELISA Kit (Cat# AAA164350)

Full Name

Mouse TGF-beta1 (Transforming Growth Factor beta1) ELISA Kit

Gene Names

Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1

Reactivity

Mouse

Pricing

$225/24 Tests (LIMIT 1) $425/48 Tests $480/96 Tests $935/2x96 Tests $1,370/3x96 Tests $1,775/4x96 Tests $1,990/5x96 Tests $3,940/10x96 Tests

Standard Curve (Sample)

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Hyaluronoglucosaminidase 1 (HYAL1), Assay Kit (Cat# AAA161344)

Full Name

Hyaluronoglucosaminidase 1 (HYAL1) Magnetic Fluorescence Assay Kit

Reactivity

Rat

Pricing

$1,200/96 Tests $2,340/2x96 Tests $3,415/3x96 Tests $4,435/4x96 Tests $4,830/5x96 Tests $8,785/10x96 Tests

What are the Benefits of ELISA Kits?

• High sensitivity and specificity: Detects even low levels of analytes with excellent accuracy.


• Quantitative results: Provides precise results of antigen and antibody concentration.


• Scalable and high throughput: Ideal for analyzing large numbers of sample at the same time.


• Easy to use: Intuitive and straightforward procedure


• Versatile applications: Ideal for immunology, cancer research, infectious disease, food safety, and more.

What is Included in an ELISA Kit?

Each ELISA kit from AAA Biotech typically contains:

• Pre-coated 96/48/24-well microplate


• Standards and/or controls


• Detection antibody


• Enzyme conjugate


• Substrate solution (e.g., TMB)


• Stop solution


• Sample diluent and wash buffer


• Detailed protocol and instructions

Why Buy ELISA Kits from AAA Biotech?

• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Wide Selection of ELISA Kits: Extensive product range covering multiple species, targets, and research areas.


• Affordable Prices: High-quality kits at competitive prices to support all research budgets.


• Over 10 Years Supporting the Research Community: A dedicated partner to researchers worldwide.


• Shipping to 100+ Countries: Reliable international delivery with tracked shipments.


• High-Quality, Validated Components: Designed for accuracy, reproducibility, and performance in research settings.


• Trusted by Researchers Globally: Used in universities, biotech labs, and research institutes around the world.


• Various Applications: Ideal for basic science, immunology, oncology, infectious disease study, and more.

FAQ

1. When should I use a competitive ELISA assay?

Competitive ELISA is ideal when the target analyte is smaller molecule (such as hormones or other short peptides or lipids/sugars) and there is unlikely to be sufficient surface or possible epitopes on the analyte/molecule to allow for two antibodies to be able to bind to it simultaneously (as would be required by an ELISA using the traditional “sandwich” design format).

2. What samples can ELISA kits be used for?

ELISA kits are compatible with a wide variety of biological samples, including serum, plasma, cell culture supernatants, urine, saliva, and tissue homogenates. Serum & plasma are routinely tested with the vast majority of kits in our catalog, but since many target analytes do not exist in these sample types, some will be further validated with various tissue homogenates or cell lysates samples. The samples ultimately tested by the manufacturing lab technician will largely depend on the target analyte’s typical location in an organism, or where its presence is of interest to the research community.

3. How long can I store my sample before using it for an ELISA assay?

Sample shelf-life ultimately depends on the intrinsic stability of the target analyte in the samples’ specific biological matrix, and also any other factors affecting it from storage (such as added preservatives and temperature). Generally:

• Short-term (up to 48 hours): Store at 2–8°C


• Long-term: Store at -20°C or -80°C for most biological samples

Avoid repeated freeze-thaw cycles to maintain sample integrity.