WB (Western Blot)
CFP10-ESAT6 protein
CFP10-ESAT6 Protein
Applications
ELISA, Western Blot
Purity
>95% as analyzed by SDS-PAGE. The concentration is 2.6mg/ml
Synonyms
CFP10-ESAT6; N/A; CFP10-ESAT6 Protein; CFP10-ESAT6 protein
Host
E Coli
Purity/Purification
>95% as analyzed by SDS-PAGE. The concentration is 2.6mg/ml
Form/Format
Purified protein solution after extensive dialysis against PBS, pH 7.4
Sequence
TEQQWNFAGI EAAASAIQGN VTSIHSLLDE GKQSLTKLAA AWGGSGSEAY QGVQQKWDAT ATELNNALQN LARTISEAGQ AMASTEGNVT GMFAGTAEMK TDAATLAQEA GNFERISGDL KTQIDQVEST AGSLQGQWRG AAGTAAQAAV VRFQEAANKQ KQELDEISTN IRQAGVQYSR ADEEQQQALS SQMGF
Applicable Applications for CFP10-ESAT6 protein
ELISA, WB (Western Blot), Antibody Production
Endotoxin
Less than 0.2 EU/ug determined by LAL test.
Species
Mycobacterium tuberculosis
Expression System
CFP10-ESAT6 fusion antigen, produced in E coli is a single non-glycosylated polypeptide chain containing 195 amino acids. A fully biologically active molecule has a molecular mass of 20.5kDa. The apparent molecular mass of the protein is approximately 21kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Preparation and Storage
Samples are stable for up to 12 months from date of receipt at -20C to -80C. It is recommended that the protein be aliquoted to optimal storage. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
WB (Western Blot)
Related Product Information for CFP10-ESAT6 protein
Mycobactenum antigen ESAT6 has been isolated from low molecular weight fractions of the shot-term culture filtrate (ST-CF) and it can easily be detected in tuberculosis patients. The export of ESAT-6 which is a poten T-cell antigen, and related proteins requires a dedicated secretory apparatus that is encoded by a group of genes, several of which also code for proteins that are recognized strongly by T cells. The ESAT-6 systems can consequently be considered as immunogenicity islands and there is mounting evidence that the equivalent genes are subject to selective pressure imposed by the immune system of the host. This antigen includes many epitopes detectable in the serum of most patients with tuberculosis (more than 90%). By the attempts to obtain the vaccine on the basis of ESAT-6, it was demonstrated that the optimization of adjuvant is very important when using the combination of dioctadecylammonium bromide and monophosphoryllipide. ESAT-6 is very potential as diagnostic for differentiation between the mycobacterial infection and BGC vaccination. The main topic of ESAT-6 using is in antibody production and in test-systems for tuberculosis elaboration. Early secretory antigen 6 (ESAT6) and cell filtrate protein 10 (CFP10) are 2 antigens secreted as a complex by the replicating Mycobacterium tuberculosis complex (MTC).
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
20.5kDa. The apparent molecular mass of the protein is approximately 21kDa observed by reducing SDS-PAGE
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Product Notes
The CFP10-ESAT6 (Catalog #AAA27924) is a Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CFP10-ESAT6 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), Antibody Production. Researchers should empirically determine the suitability of the CFP10-ESAT6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TEQQWNFAGI EAAASAIQGN VTSIHSLLDE GKQSLTKLAA AWGGSGSEAY QGVQQKWDAT ATELNNALQN LARTISEAGQ AMASTEGNVT GMFAGTAEMK TDAATLAQEA GNFERISGDL KTQIDQVEST AGSLQGQWRG AAGTAAQAAV VRFQEAANKQ KQELDEIST NIRQAGVQY SRADEEQQQ ALSSQMGF. It is sometimes possible for the material contained within the vial of "CFP10-ESAT6, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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