FGF10 active protein

FGF10 Protein, Human, Recombinant

Cat. Num. AAA257898

• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF10; N/A; FGF10 Protein, Human, Recombinant; Human FGF10/KGF2 Protein; KGF2 Protein, Human; fibroblast growth factor 10; FGF10 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 1mM DTT, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln38-Ser208

• Species: Human
• Activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The EC50 for this effect is typically 2-14ng/mL.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human FGF10 (NP_004456.1)(Gln38-Ser208) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The EC50 for this effect is typically 2-14 ng/mL.)

SDS-PAGE

Related Product Information for FGF10 active protein

Background: Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.

References

Sekine K, et al. (1999) Fgf10 is essential for limb and lung formation. Nat Genet. 21(1): 138-41.Ohuchi H, et al. (2000) FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development. Biochem Biophys Res Commun. 277(3): 643-9.Bellusci S, et al. (1997) Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development. 124(23): 4867-78.

NCBI and Uniprot Product Information

• NCBI GI #: 4758360
• NCBI GeneID: 2255
• NCBI Accession #: NP_004456.1
• NCBI GenBank Nucleotide #: NM_004465.1
• UniProt Accession #: O15520; Q6FHT6; Q96P59
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• UniProt Protein Name: Fibroblast growth factor 10
• UniProt Gene Name: FGF10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_HUMAN