Bioactivity
(Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is )
IL-10 R alpha active protein
Recombinant IL-10 R alpha
Gene Names
IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
Applications
SDS-Page
Purity
> 90% by SDS - PAGE.
Synonyms
IL-10 R alpha; N/A; Recombinant IL-10 R alpha; IL10RA; Interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL-10R,IL-10R1; IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; Interleukin-10receptor subunit 1; IL-10R subunit 1; IL-10 R alpha active protein
Host
Baculovirus
Purity/Purification
> 90% by SDS - PAGE.
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol.
Concentration
0.2mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
22-235aa
Sequence
HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSYDLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILGKIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS CVQVKPSVAS RSNKGMWSKEECISLTRQYF TVTN
Sequence Length
578
Applicable Applications for IL-10 R alpha active protein
Bioactivity, SDS-PAGE
Tag
His-Tag
Biological Activity
Measured by its ability to inhibit proliferation using MC/9 mouse mast cells. The ED50 for this effect is <= 300 ng/ml with Human IL-10.
Predicted nterm
His 22
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C.Avoidrepeated freezing and thawing cycles.
Bioactivity
(Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is )
Bioactivity
(Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is )
SDS-PAGE
(3ug by SDS-PAGE under reducing conditionandvisualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing conditionandvisualized by coomassie blue stain)
Related Product Information for IL-10 R alpha active protein
IL-10 R alpha, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Recombinant human IL-10 R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for IL-10 R alpha active protein
References
Josephson K, et al. (2001) Acta Crystallogr D Biol Crystallogr. 57(Pt 12):1908-11.;;Usacheva A, etal. (2002) J Immunol. 169(3):1302-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25.2kDa (220aa)
NCBI Official Full Name
interleukin-10 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 10 receptor subunit alpha
NCBI Official Symbol
IL10RA
NCBI Official Synonym Symbols
CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
NCBI Protein Information
interleukin-10 receptor subunit alpha
UniProt Protein Name
Interleukin-10 receptor subunit alpha
UniProt Gene Name
IL10RA
UniProt Synonym Gene Names
IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
UniProt Entry Name
I10R1_HUMAN
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Product Notes
The IL-10 R alpha il10ra (Catalog #AAA48321) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-235aa. AAA Biotech's IL-10 R alpha can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the IL-10 R alpha il10ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSY DLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILG KIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS CVQVKPSVAS RSNKGMWSKE ECISLTRQYF TVTNPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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