Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Recombinant Protein | ATP2A2 recombinant protein

Recombinant Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Cat. Num. AAA113752

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; N/A; Recombinant Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2A2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 314-756aa; Partial
• Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNM

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ATP2A2 recombinant protein

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.

Product Categories/Family for ATP2A2 recombinant protein

Cardiovascular

References

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Genet. 36:40-45(2004) Identification of a new SERCA2 splice variant regulated during monocytic differentiation.Gelebart P., Martin V., Enouf J., Papp B.Biochem. Biophys. Res. Commun. 303:676-684(2003) TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump Serca2b and is necessary for collagen type I synthesis.Stefanovic B., Stefanovic L., Schnabl B., Bataller R., Brenner D.A.Mol. Cell. Biol. 24:1758-1768(2004) Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging.Xu S., Ying J., Jiang B., Guo W., Adachi T., Sharov V., Lazar H., Menzoian J., Knyushko T.V., Bigelow D., Schoeneich C., Cohen R.A.Am. J. Physiol. 290:H2220-H2227(2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.Cell 127:635-648(2006) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.Mol. Cell 31:438-448(2008) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) The anti-apoptotic protein HAX-1 interacts with SERCA2 and regulates its protein levels to promote cell survival.Vafiadaki E., Arvanitis D.A., Pagakis S.N., Papalouka V., Sanoudou D., Kontrogianni-Konstantopoulos A., Kranias E.G.Mol. Biol. Cell 20:306-318(2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) POST, partner of stromal interaction molecule 1 (STIM1) , targets STIM1 to multiple transporters.Krapivinsky G., Krapivinsky L., Stotz S.C., Manasian Y., Clapham D.E.Proc. Natl. Acad. Sci. U.S.A. 108:19234-19239(2011) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) Spectrum of novel ATP2A2 mutations in patients with Darier's disease.Sakuntabhai A., Burge S., Monk S., Hovnanian A.Hum. Mol. Genet. 8:1611-1619(1999) ATP2A2 mutations in Darier's disease variant cutaneous phenotypes are associated with missense mutations, but neuropsychiatric features are independent of mutation class.Ruiz-Perez V.L., Carter S.A., Healy E., Todd C., Rees J.L., Steijlen P.M., Carmichael A.J., Lewis H.M., Hohl D., Itin P., Vahlquist A., Gobello T., Mazzanti C., Reggazini R., Nagy G., Munro C.S., Strachan T.Hum. Mol. Genet. 8:1621-1630(1999) ATP2A2 mutations in Darier's disease and their relationship to neuropsychiatric phenotypes.Jacobsen N.J.O., Lyons I., Hoogendoorn B., Burge S., Kwok P.-Y., O'Donovan M.C., Craddock N., Owen M.J.Hum. Mol. Genet. 8:1631-1636(1999) Mutations in ATP2A2, encoding a Ca2+ pump, cause Darier disease.Sakuntabhai A., Ruiz-Perez V., Carter S., Jacobsen N., Burge S., Monk S., Smith M., Munro C.S., O'Donovan M.C., Craddock N., Kucherlapati R., Rees J.L., Owen M.J., Lathrop G.M., Monaco A.P., Strachan T., Hovnanian A.Nat. Genet. 21:271-277(1999) Acrokeratosis verruciformis of Hopf is caused by mutation in ATP2A2 evidence that it is allelic to Darier's disease.Dhitavat J., Macfarlane S., Dode L., Leslie N., Sakuntabhai A., MacSween R., Saihan E., Hovnanian A.J. Invest. Dermatol. 120:229-232(2003) Ca2+-ATPases in non-failing and failing heart evidence for a novel cardiac sarco/endoplasmic reticulum Ca2+-ATPase 2 isoform (SERCA2c) .Dally S., Bredoux R., Corvazier E., Andersen J.P., Clausen J.D., Dode L., Fanchaouy M., Gelebart P., Monceau V., Del Monte F., Gwathmey J.K., Hajjar R., Chaabane C., Bobe R., Raies A., Enouf J.Biochem. J. 395:249-258(2006) Three-base deletion mutation c.120_122delGTT in ATP2A2 leads to the unique phenotype of comedonal Darier disease.Tsuruta D., Akiyama M., Ishida-Yamamoto A., Imanishi H., Mizuno N., Sowa J., Kobayashi H., Ishii M., Kurokawa I., Shimizu H.Br. J. Dermatol. 162:687-689(2010) +Additional computationally mapped references.<p>Provides general information on the entry.

NCBI and Uniprot Product Information

• NCBI GI #: 4502285
• NCBI GeneID: 488
• NCBI Accession #: NP_001672.1
• NCBI GenBank Nucleotide #: NM_001681.3
• UniProt Accession #: P16615; P16614; Q86VJ2; A6NDN7; B4DF05
• Molecular Weight: 52.3 kDa
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2; SR Ca(2+)-ATPase 2
• UniProt Entry Name: AT2A2_HUMAN