Standard Curve (Sample)
CytoSelect Leukocyte Transmigration Assay Assay Kit
CytoSelect Leukocyte Transmigration Assay
Synonyms
CytoSelect Leukocyte Transmigration Assay; N/A; CytoSelect Leukocyte Transmigration Assay assay kit
Preparation and Storage
LeukoTracker Solution and TNFa should be removed from the kit and stored at -20 degree C immediately. Store all other components at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CytoSelect Leukocyte Transmigration Assay assay kit
Background: Leukocyte extravasation into perivascular tissue plays a key role in inflammatory diseases. This recruitment requires leukocyte interaction with vascular endothelium and consists of multiple, consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration (Figure 1). This multistep paradigm is realized by sequential activation-dependent interactions between endothelial cell adhesion molecules and their specific ligands on leukocytes. The first step of transient adhesion and rolling is known to be mediated by an interaction of leukocyte or endothelial cell selectins and their oligosaccharide-bearing ligands. Arrest and firm adhesion of leukocytes to endothelium is dependent on the activation of beta2 integrins like Mac-1 or LFA-1 on the leukocyte cell surface, followed by interaction with endothelial cell proteins belonging to the Ig superfamily such as ICAM-1. CytoSelect™ Leukocyte Transmigration Assay provides a robust system for the quantitative determination of leukocyte-endothelium interactions and transmigrations. The kit contains sufficient reagents for the evaluation of 24 assays in a 24-well plate format.
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References
1. Carlos T. M and Harlan J. M. (1994) Blood 84, 2068-101.
2. Meldrum, D. R. (1998) Am. J. Physiol. 274, R577-R595.
1. Zhao, L. et al. (2008). CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289.
2. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell growth. Cancer Res. 68:6752-6761.
2. Meldrum, D. R. (1998) Am. J. Physiol. 274, R577-R595.
1. Zhao, L. et al. (2008). CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289.
2. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell growth. Cancer Res. 68:6752-6761.
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Product Notes
The CytoSelect Leukocyte Transmigration Assay (Catalog #AAA44523) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect Leukocyte Transmigration Assay, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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