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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

POU5F1, Monoclonal Antibody (Cat# AAA26362)

Full Name

POU5F1 (POU Class 5 Homeobox 1, MGC22487, OCT3, OCT4, OTF3, OTF4) (FITC)

Applications

EIA, IF, WB

Purity

Purified

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-PKP2 antibody (AAA19770).Overlay histogram showing U87 cells stained with AAA19770 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKP2 Antibody (AAA19770, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PKP2 using anti-PKP2 antibody (AAA19770) and anti-Beta Tubulin antibody (M01857-3).PKP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKP2 antigen affinity purified polyclonal antibody (#AAA19770) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKP2 at approximately 92 kDa. The expected band size for PKP2 is at 97 kDa.)

PKP2, Polyclonal Antibody (Cat# AAA19770)

Full Name

Anti-PKP2 Antibody Picoband

Gene Names

PKP2; ARVD9

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-PRDM15 antibody (AAA19987).Overlay histogram showing A431 cells stained with AAA19987 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM15 Antibody (AAA19987, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PRDM15 using anti-PRDM15 antibody (AAA19987).PRDM15 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM15 antigen affinity purified polyclonal antibody (#AAA19987) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (62 kDa.)

PRDM15, Polyclonal Antibody (Cat# AAA19987)

Full Name

Anti-PRDM15 Antibody Picoband

Gene Names

PRDM15; PFM15; ZNF298; C21orf83

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation of GART transfected lysate using GART monoclonal antibody and Protein A Magnetic Bead and immunoblotted with GART rabbit polyclonal antibody.)

GART, Monoclonal Antibody (Cat# AAA25402)

Full Name

GART (Trifunctional Purine Biosynthetic Protein Adenosine-3, PGFT, PRGS, MGC47764) (HRP)

Gene Names

GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

NMI, Monoclonal Antibody (Cat# AAA25473)

Full Name

NMI (N-myc-interactor, Nmi, N-myc and STAT Interactor) (HRP)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA24935)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (Biotin)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

UBTF, Monoclonal Antibody (Cat# AAA26370)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (FITC)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

CMTM6, Polyclonal Antibody (Cat# AAA29213)

Full Name

CMTM6 Polyclonal Antibody

Gene Names

CMTM6; CKLFSF6; PRO2219

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

Inducible nitric oxide synthase, ELISA Kit (Cat# AAA16142)

Full Name

Mouse Inducible nitric oxide synthase ELISA Kit

Gene Names

Inos; bs36h12.y1; CG11143; DmelCG11143; INOS

Reactivity

Mouse

Pricing

Complement 5 a (C5a), ELISA Kit (Cat# AAA12868)

Full Name

Porcine Complement 5 a (C5a) ELISA Kit

Reactivity

Porcine

Pricing

Heparin, ELISA Kit (Cat# AAA17218)

Full Name

Porcine Heparin ELISA Kit

Reactivity

Porcine

Pricing

Brain Natriuretic Peptide (BNP), ELISA Kit (Cat# AAA12886)

Full Name

Mouse Brain Natriuretic Peptide (BNP) ELISA Kit

Reactivity

Mouse

Pricing

Cyclin B (CCNB), ELISA Kit (Cat# AAA23044)

Full Name

Human Cyclin B (CCNB) ELISA Kit

Gene Names

CycB; 2g; 3510; CG3510; chr2R:18312808..18312907; cyc B; Cyc B; cyc-B; CYC-B; cycb; cycB; Cycb; CYCB; cyclin B; cyclinB; DmcycB; DmelCG3510; mAbF2F4

Reactivity

Human

Pricing

Lysophosphatidylcholine, ELISA Kit (Cat# AAA20998)

Full Name

General Lysophosphatidylcholine (LPC) ELISA Kit

Reactivity

General

Pricing

N-terminal pro-brain natriuretic peptide (NT-proBNP), ELISA Kit (Cat# AAA18183)

Full Name

Canine N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit

Reactivity

Dog

Pricing

Matrix Metalloproteinase 1, ELISA Kit (Cat# AAA17672)

Full Name

Rat Matrix Metalloproteinase 1 ELISA Kit

Gene Names

Mmp1; CG4859; dm1-MMP; Dm1-MMP; DmelCG4859; dmmp1; dMMP1; l(2)k04809; Mmp 1; MMP-1; mmp1; MMp1; MMP1

Reactivity

Rat

Pricing

Ethoxyresorufin-O-deethylase (EROD), ELISA Kit (Cat# AAA18981)

Full Name

Fish Ethoxyresorufin-O-deethylase (EROD) ELISA Kit

Reactivity

Fish

Pricing

N-Terminal Gasdermin D, ELISA Kit (Cat# AAA27432)

Full Name

Mouse N-Terminal Gasdermin D ELISA Kit

Reactivity

Mouse

Pricing

plasmin-antiplasmin complex, PAP, ELISA Kit (Cat# AAA15415)

Full Name

Rat plasmin-antiplasmin complex, PAP ELISA Kit

Reactivity

Rat

Pricing

Olfactomedin 4 (OLFM4), ELISA Kit (Cat# AAA20557)

Full Name

Human Olfactomedin 4 (OLFM4) ELISA Kit

Gene Names

OLFM4; GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1

Reactivity

Human

Pricing

N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP), ELISA Kit (Cat# AAA12406)

Full Name

Mouse N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

Gene Names

NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF

Reactivity

Mouse

Pricing

Follicle Stimulating Hormone (FSH), ELISA Kit (Cat# AAA12415)

Full Name

Chicken Follicle Stimulating Hormone (FSH) ELISA Kit

Reactivity

Chicken

Pricing

Visceral Adipose Specific Serine Protease Inhibitor, ELISA Kit (Cat# AAA17535)

Full Name

Human Visceral Adipose Specific Serine Protease Inhibitor ELISA Kit

Reactivity

Human

Pricing

Argininosuccinate lyase (ASL), ELISA Kit (Cat# AAA27299)

Full Name

Human Argininosuccinate lyase (ASL) ELISA Kit

Reactivity

Human

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP90AA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HSP90AA1, Polyclonal Antibody (Cat# AAA22176)

Full Name

HSP90AA1 Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

erythropoietin, ELISA Kit (Cat# AAA15024)

Full Name

Dog Erythropoietin, EPO ELISA Kit

Reactivity

Dog

Pricing

Hypoxia-Inducible Factor 1alpha, ELISA Kit (Cat# AAA23687)

Full Name

Human Hypoxia-Inducible Factor 1alpha Antibody ELISA Kit

Reactivity

Human

Pricing

Dihydrotestosterone, DHT, ELISA Kit (Cat# AAA14999)

Full Name

Canine Dihydrotestosterone, DHT ELISA Kit

Reactivity

Dog

Pricing

Hypoxia-inducible gene 2 protein, ELISA Kit (Cat# AAA17318)

Full Name

Human Hypoxia-inducible gene 2 protein ELISA Kit

Reactivity

Human

Pricing

Myosin Light Chain Kinase (MLCK), ELISA Kit (Cat# AAA12460)

Full Name

Mouse Myosin Light Chain Kinase (MLCK) ELISA Kit

Gene Names

MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083

Reactivity

Mouse

Pricing

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Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)

SMARCB1, Monoclonal Antibody (Cat# AAA25253)

Full Name

SMARCB1 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily B Member 1, BRG1-associated Factor 47, BAF47, Integrase Interactor 1 Protein, SNF5 Homolog, hSNF5, BAF47, INI1, SNF5L1) (FITC)

Gene Names

SMARCB1; RDT; CSS3; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Complement C1s subcomponent (C1S), ELISA Kit (Cat# AAA27341)

Full Name

Mouse Complement C1s subcomponent (C1S) ELISA Kit

Reactivity

Mouse

Pricing

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WB (Western Blot) (Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M02), clone 1D11.Lane 1: TRIM28 transfected lysate (Predicted MW: 88.5 KDa).Lane 2: Non-transfected lysate.)

TRIM28, Monoclonal Antibody (Cat# AAA26579)

Full Name

TRIM28 (Tripartite Motif-Containing 28, FLJ29029, KAP1, RNF96, TF1B, TIF1B) (PE)

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Applications

EIA, IHC, WB

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)

ADRM1, Monoclonal Antibody (Cat# AAA25308)

Full Name

ADRM1 (GP110, Proteasomal Ubiquitin Receptor ADRM1, 110kD Cell Membrane Glycoprotein, Adhesion-regulating Molecule 1, Proteasome Regulatory Particle Non-ATPase 13, Rpn13 Homolog, MGC29536) (HRP)

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-Dynamin 1 antibody (AAA19160).Overlay histogram showing U20S cells stained with AAA19160 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1 Antibody (AAA19160,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Dynamin 1, Polyclonal Antibody (Cat# AAA19160)

Full Name

Anti-Dynamin 1 Picoband antibody

Gene Names

DNM1; DNM; EIEE31

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, FC/FACS, IHC, ICC, WB

Pricing

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Application Data (Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.)

STAT5B, Monoclonal Antibody (Cat# AAA26337)

Full Name

STAT5B (Signal Transducer and Activator of Transcription 5B, STAT5) (FITC)

Gene Names

STAT5B; STAT5

Applications

IF, IHC, WB

Purity

Purified

Pricing

Angiotensin 1-7, ELISA Kit (Cat# AAA15332)

Full Name

Rat angiotensin 1-7, Ang1-7 ELISA Kit

Gene Names

ANGPT1; AGP1; AGPT; ANG1

Reactivity

Rat

Pricing

Glutathione peroxidase, ELISA Kit (Cat# AAA16864)

Full Name

Bovine Glutathione peroxidase ELISA Kit

Gene Names

GPX3; GPx-P; GSHPx-3; GSHPx-P

Reactivity

Bovine

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (34.21kD).)

SMAD5, Monoclonal Antibody (Cat# AAA25842)

Full Name

SMAD5 (Mothers Against Decapentaplegic Homolog 5, Mothers Against DPP Homolog 5, MAD Homolog 5, Mad5, SMAD Family Member 5, SMAD 5, Smad5, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323) (PE)

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

follistatin-like 1, ELISA Kit (Cat# AAA15091)

Full Name

Human Follistatin Like Protein 1, FSTL1 ELISA Kit

Gene Names

FSTL1; FRP; FSL1; MIR198

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)

KV4.3, Polyclonal Antibody (Cat# AAA29939)

Full Name

KV4.3 Antibody

Gene Names

KCND3; KV4.3; SCA19; SCA22; KCND3L; KCND3S; KSHIVB

Reactivity

Human, Mouse

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified.

Pricing

Interferon-induced protein with tetratricopeptide repeats 3, IFIT3, ELISA Kit (Cat# AAA22524)

Full Name

Human Interferon-induced protein with tetratricopeptide repeats 3, IFIT3 ELISA Kit

Gene Names

IFIT3; P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; CIG-49; GARG-49

Reactivity

Human

Pricing

Cytochrome Oxidase 4, ELISA Kit (Cat# AAA16379)

Full Name

Mouse Cytochrome Oxidase 4 ELISA Kit

Reactivity

Mouse

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Smad1, Polyclonal Antibody (Cat# AAA28413)

Full Name

[KO Validated] Smad1 Rabbit pAb

Reactivity

Human

Applications

WB, IHC, IF, ICC

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)

Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)

Full Name

[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb

Gene Names

NR1D2; RVR; BD73; EAR-1R

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ICC

Purity

Affinity purification

Pricing

Clumping factor A (clfA), partial, Recombinant Protein (Cat# AAA18783)

Full Name

Recombinant Staphylococcus aureus Clumping factor A (clfA), partial

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (AAA19967).Overlay histogram showing U937 cells stained with AAA19967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (AAA19967).Syntaxin 18/STX18 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (#AAA19967) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.)

Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)

Full Name

Anti-Syntaxin 18/STX18 Antibody Picoband

Gene Names

STX18; Ufe1

Reactivity

Human

Applications

EIA, FC/FACS, IF, IHC, WB

Purity

Immunogen affinity purified.

Pricing

Alpha-hemolysin, Recombinant Protein (Cat# AAA18739)

Full Name

Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Uterine Fibroids Tissue)

Chymase 1, Mast Cell (CMA1), Polyclonal Antibody (Cat# AAA20093)

Full Name

Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)

Gene Names

Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5

Reactivity

Human, Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti- ETF/TEAD2 antibody (AAA19325).Overlay histogram showing Hela cells stained withAAA19325 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ETF/TEAD2 Antibody (AAA19325, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ETF/TEAD2, Polyclonal Antibody (Cat# AAA19325)

Full Name

Anti-ETF/TEAD2 Antibody

Gene Names

TEAD2; ETF; TEF4; TEF-4; TEAD-2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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