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Interferon beta, IFN-beta, ELISA Kit (Cat# AAA19013)

Full Name

Cat Interferon beta, IFN-beta ELISA Kit

Gene Names

IFNB1; IFB; IFF; IFNB; IFN-beta

Reactivity

Cat

Pricing

hyaluronoglucosaminidase 1, ELISA Kit (Cat# AAA15428)

Full Name

Human Hyaluronidase-1, HYAL1 ELISA Kit

Gene Names

HYAL1; NAT6; LUCA1; HYAL-1

Reactivity

Human

Pricing

Annexin A2 antibody, ELISA Kit (Cat# AAA27143)

Full Name

Human Annexin A2 antibody ELISA Kit

Reactivity

Human

Pricing

Gamma-Aminobutyric Acid (gABA), ELISA Kit (Cat# AAA22830)

Full Name

General Gamma-Aminobutyric Acid (gABA) ELISA Kit

Reactivity

General

Pricing

Mullerian hormone, ELISA Kit (Cat# AAA22607)

Full Name

Canine Anti-Mullerian hormone (AMH) ELISA Kit

Reactivity

Canine

Pricing

Synaptosomal-associated protein 25 (SNAP25), ELISA Kit (Cat# AAA11353)

Full Name

Human Synaptosomal-associated protein 25 (SNAP25) ELISA Kit

Gene Names

Snap25; SNAP-25B; SNAP-25a

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-OTUB1 antibody (AAA19813).Overlay histogram showing MCF-7 cells stained with AAA19813 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTUB1 Antibody (AAA19813, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of OTUB1 using anti-OTUB1 antibody (AAA19813).OTUB1 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUB1 antigen affinity purified polyclonal antibody (#AAA19813) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OTUB1 at approximately 36 kDa. The expected band size for OTUB1 is at 31 kDa.)

OTUB1, Polyclonal Antibody (Cat# AAA19813)

Full Name

Anti-OTUB1 Antibody Picoband

Gene Names

OTUB1; OTB1; OTU1; HSPC263

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

Creatine Kinase MB isoenzyme, ELISA Kit (Cat# AAA16749)

Full Name

Porcine Creatine Kinase MB isoenzyme ELISA Kit

Gene Names

Ckm; Ckmm

Reactivity

Porcine

Pricing

IF (Immunofluorescence) (Figure 10. IF analysis of H2AFY/MACROH2A1 using anti-H2AFY/MACROH2A1 antibody (AAA19821) and anti-Beta Tubulin antibody (M01857-3).H2AFY/MACROH2A1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat thymus tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY/MACROH2A1 antigen affinity purified polyclonal antibody (#AAA19821) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for H2AFY/MACROH2A1 at approximately 39 kDa. The expected band size for H2AFY/MACROH2A1 is at 40 kDa.)

H2AFY/MACROH2A1, Polyclonal Antibody (Cat# AAA19821)

Full Name

Anti-H2AFY/MACROH2A1 Antibody Picoband

Gene Names

H2AFY; H2A.y; H2A/y; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

Heat Shock Protein 70, ELISA Kit (Cat# AAA16239)

Full Name

Rat Heat Shock Protein 70 ELISA Kit

Reactivity

Rat

Pricing

N-terminal propeptide of Collagen alpha-1(III) chain, ELISA Kit (Cat# AAA23174)

Full Name

Human N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit

Reactivity

Human

Pricing

Angiopoietin Like Protein 4, ELISA Kit (Cat# AAA17547)

Full Name

Mouse Angiopoietin Like Protein 4 ELISA Kit

Reactivity

Mouse

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse heart using DES Polyclonal Antibody at dilution of 1:100 (40x lens).)

DES, Polyclonal Antibody (Cat# AAA22175)

Full Name

DES Polyclonal Antibody

Gene Names

DES; CSM1; CSM2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

Granzyme B (Gzms-B), ELISA Kit (Cat# AAA12701)

Full Name

Porcine Granzyme B (Gzms-B) ELISA Kit

Gene Names

GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

Reactivity

Porcine

Pricing

Coenzyme Q10 (CoQ10), ELISA Kit (Cat# AAA27291)

Full Name

Rat Coenzyme Q10 (CoQ10) ELISA Kit

Reactivity

Rat

Pricing

Follicle Stimulating Hormone (FSH), ELISA Kit (Cat# AAA12465)

Full Name

Mouse Follicle Stimulating Hormone (FSH) ELISA Kit

Reactivity

Mouse

Pricing

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IP (Immunoprecipitation) (Figure 10. Immunoprecipitating TNS3 in U251 whole cell lysate .Western blot analysis of TNS3 using anti-TNS3 antibody (AAA19884).Lane 1: U251 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-TNS3 antibody in U251 whole cell lysate,Lane 3: anti-TNS3 antibody (2ug) + U251 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (AAA19884) at a dilution of 0.5ug/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.)

IF (Immunofluorescence) (Figure 8. IF analysis of TNS3 using anti-TNS3 antibody (AAA19884).TNS3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (#AAA19884) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.)

TNS3, Polyclonal Antibody (Cat# AAA19884)

Full Name

Anti-TNS3 Antibody Picoband

Gene Names

TNS3; TEM6; TENS1

Reactivity

Human

Applications

WB, IHC, ICC, IF, IP, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

3alpha-Androstanediol, ELISA Kit (Cat# AAA17283)

Full Name

Rat 3alpha-Androstanediol ELISA Kit

Reactivity

Rat

Pricing

Deoxyribonuclease I, ELISA Kit (Cat# AAA16279)

Full Name

Mouse Deoxyribonuclease I ELISA Kit

Gene Names

DNASE1; DNL1; DRNI

Reactivity

Mouse

Pricing

thiopurine S-methyltransferase, ELISA Kit (Cat# AAA18322)

Full Name

Human thiopurine S-methyltransferase, TPMT ELISA Kit

Reactivity

Human

Pricing

Protectin, ELISA Kit (Cat# AAA16809)

Full Name

Bovine Protectin ELISA Kit

Gene Names

CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

Reactivity

Bovine

Pricing

heat shock protein 72 (Hsp-72), ELISA Kit (Cat# AAA22458)

Full Name

Human heat shock protein 72 (Hsp-72) ELISA Kit

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103

Reactivity

Human

Pricing

lactate dehydrogenase A, ELISA Kit (Cat# AAA18113)

Full Name

Human lactate dehydrogenase A, LDHA ELISA Kit

Gene Names

LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P

Reactivity

Human

Pricing

apolipoprotein A1 (Apo-A1), ELISA Kit (Cat# AAA15051)

Full Name

Monkey apolipoprotein A1, Apo-A1 ELISA Kit

Reactivity

Monkey

Pricing

Phosphorylated tau 181, ELISA Kit (Cat# AAA17095)

Full Name

Mouse Phosphorylated tau 181 ELISA Kit

Reactivity

Mouse

Pricing

Scrapie-responsive protein 1 (Scrg1), Recombinant Protein (Cat# AAA18660)

Full Name

Recombinant Mouse Scrapie-responsive protein 1 (Scrg1)

Gene Names

Scrg1; AW124307

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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WB (Western Blot) (Western Blot analysis of NDUFV2 expression in transfected 293T cell line by NDUFV2 monoclonal antibody. Lane 1: NDUFV2 transfected lysate (Predicted MW: 27.4kD). Lane 2: Non-transfected lysate.)

NDUFV2, Monoclonal Antibody (Cat# AAA24286)

Full Name

NDUFV2 (NADH Dehydrogenase [Ubiquinone] Flavoprotein 2, Mitochondrial, NADH-ubiquinone Oxidoreductase 24kD Subunit) (AP)

Gene Names

NDUFV2; CI-24k

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Angiotensin I Converting Enzyme, ELISA Kit (Cat# AAA16351)

Full Name

Human Angiotensin I Converting Enzyme ELISA Kit

Gene Names

ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3

Reactivity

Human

Pricing

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Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)

PAK1, Monoclonal Antibody (Cat# AAA24301)

Full Name

PAK1 (Serine/Threonine-protein Kinase PAK 1, Alpha-PAK, p21-activated Kinase 1, PAK-1, p65-PAK, MGC130000, MGC130001) (AP)

Gene Names

PAK1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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RAB7, Monoclonal Antibody (Cat# AAA30183)

Full Name

RAB7 Antibody

Gene Names

RAB7A; RAB7; PRO2706

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, FC

Purity

ProA affinity purified

Pricing

Protein tyrosine phosphatase, ELISA Kit (Cat# AAA17131)

Full Name

Chicken Protein tyrosine phosphatase ELISA Kit

Reactivity

Chicken

Pricing

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])

TAF11, Monoclonal Antibody (Cat# AAA26086)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (APC)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

IF, IHC, WB

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26863)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 650)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

WB, IHC, IF

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

alpha Melanocyte Stimulating Hormone, ELISA Kit (Cat# AAA16880)

Full Name

Bovine alpha Melanocyte Stimulating Hormone ELISA Kit

Reactivity

Bovine

Pricing

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), Recombinant Protein (Cat# AAA18790)

Full Name

Recombinant Human Receptor-interacting serine/threonine-protein kinase 3 (RIPK3)

Gene Names

RIPK3; RIP3

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-Ch TOG/CKAP5 antibody (AAA19386).Overlay histogram showing A549 cells stained with AAA19386 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ch TOG/CKAP5 Antibody (AAA19386, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)