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Smoothened homolog (SMO), ELISA Kit (Cat# AAA27318)

Full Name

Human Smoothened homolog (SMO) ELISA Kit

Gene Names

SMO; Gx; SMOH; FZD11

Reactivity

Human

Pricing

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WB (Western Blot) (DCUN1D1 monoclonal antibody, Western Blot analysis of DCUN1D1 expression in HepG2.)

DCUN1D1, Monoclonal Antibody (Cat# AAA24778)

Full Name

DCUN1D1 (DCN1-like Protein 1, DCUN1 Domain-containing Protein 1, Defective in Cullin Neddylation Protein 1-like Protein 1, Squamous Cell Carcinoma-related Oncogene, DCUN1L1, RP42, SCCRO) (Biotin)

Gene Names

DCUN1D1; RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Syntaxin binding protein 1 (STXBP1), ELISA Kit (Cat# AAA27333)

Full Name

Human Syntaxin binding protein 1 (STXBP1) ELISA Kit

Gene Names

STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1

Reactivity

Human

Pricing

Alpha Smooth Muscle Actin, ELISA Kit (Cat# AAA16593)

Full Name

Human Alpha Smooth Muscle Actin ELISA Kit

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human

Pricing

C-telopeptide of type II collagen, CTX-II, ELISA Kit (Cat# AAA11224)

Full Name

Rat C-telopeptide of type II collagen, CTX-II ELISA Kit

Reactivity

Rat

Pricing

Microtubule-associated protein tau (Mapt), Recombinant Protein (Cat# AAA18504)

Full Name

Recombinant Mouse Microtubule-associated protein tau (Mapt)

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

Glucose-6-phosphate isomerase, Recombinant Protein (Cat# AAA15945)

Full Name

Recombinant Human Glucose-6-phosphate isomerase

Gene Names

GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

immunoglobulin heavy constant gamma 2 (G2m marker) (IGHG2), ELISA Kit (Cat# AAA22763)

Full Name

Human immunoglobulin heavy constant gamma 2 (G2m marker) (IGHG2) ELISA Kit

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-Aurora A/AURKA antibody (AAA19392).Overlay histogram showing HepG2 cells stained with AAA19392 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aurora A/AURKA Antibody (AAA19392, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Aurora A/AURKA, Polyclonal Antibody (Cat# AAA19392)

Full Name

Anti-Aurora A/AURKA Antibody Picoband

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47

Reactivity

Human

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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COVID 19 Nucleoprotein (N) / Spike Glycoprotein (S) Antibody Immunochromatography Coronavirus, Assay Kit (Cat# AAA27035)

Full Name

Human SARS-CoV-2 Nucleoprotein (N) / Spike Glycoprotein (S) Antibody Immunochromatography Assay Kit

Pricing

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FCM (Flow Cytometry) (Figure 14. Flow Cytometry analysis of 293T cells using anti-STAG2 antibody (AAA19795).Overlay histogram showing 293T cells stained with AAA19795 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG2 Antibody (AAA19795, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of STAG2 using anti-STAG2 antibody (AAA19795) and anti-Tubulin Alpha antibody (M03989-3).STAG2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SiHa whole cell lysates,Lane 3: huamn RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA2/STAG2 antigen affinity purified polyclonal antibody (#AAA19795) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SA2/STAG2 at approximately 141 kDa. The expected band size for SA2/STAG2 is at 141 kDa.)

SA2/STAG2, Polyclonal Antibody (Cat# AAA19795)

Full Name

Anti-SA2/STAG2 Antibody Picoband

Gene Names

STAG2; SA2; SA-2; SCC3B; bA517O1.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines.)

HADHB, Polyclonal Antibody (Cat# AAA21286)

Full Name

Anti-HADHB Antibody

Gene Names

HADHB; ECHB; MTPB; MSTP029; TP-BETA

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA25347)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (HRP)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)

EIF4G1, Monoclonal Antibody (Cat# AAA25382)

Full Name

EIF4G1 (Eukaryotic Translation Initiation Factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220, EIF4G1, EIF4F, EIF4G, EIF4GI) (HRP)

Gene Names

EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA25474)

Full Name

NODAL (Nodal Homolog, MGC138230) (HRP)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

MUTYH, Monoclonal Antibody (Cat# AAA24872)

Full Name

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (Biotin)

Gene Names

MUTYH; MYH

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in NIH/3T3.)

VAX1, Monoclonal Antibody (Cat# AAA25884)

Full Name

VAX1 (Ventral Anterior Homeobox 1, MCOPS11) (PE)

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 2 ug/ml])

PDK2, Monoclonal Antibody (Cat# AAA26462)

Full Name

PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2, PDHK2) (HRP)

Gene Names

PDK2; PDHK2; PDKII

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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RbAP48, Monoclonal Antibody (Cat# AAA30415)

Full Name

RbAP48 Antibody

Gene Names

RBBP4; NURF55; RBAP48; lin-53

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

SFPQ, Monoclonal Antibody (Cat# AAA30435)

Full Name

SFPQ Antibody

Gene Names

SFPQ; PSF; POMP100; PPP1R140

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

ProA affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

CDK7, Monoclonal Antibody (Cat# AAA30226)

Full Name

CDK7 Antibody

Gene Names

CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using HMGN1 antibody.)

HMGN1, Polyclonal Antibody (Cat# AAA29811)

Full Name

HMGN1 Polyclonal Antibody

Gene Names

HMGN1; HMG14

Reactivity

Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

ANG I, Polyclonal Antibody (Cat# AAA29355)

Full Name

ANG I Polyclonal Antibody

Gene Names

ANG; ALS9; HEL168; RNASE4; RNASE5

Reactivity

Human

Applications

Immunohistochemistry

Pricing

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Smad5, Polyclonal Antibody (Cat# AAA31331)

Full Name

Phospho-Smad5 (Ser463/Ser465) Antibody

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

N-terminal propeptide of Collagen alpha-1(III) chain, ELISA Kit (Cat# AAA17676)

Full Name

Human N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit

Reactivity

Human

Pricing

Citrulline, ELISA Kit (Cat# AAA16165)

Full Name

Human Citrulline ELISA Kit

Reactivity

Human

Pricing

Tau Protein, ELISA Kit (Cat# AAA16188)

Full Name

Mouse Tau Protein ELISA Kit

Gene Names

tau; BcDNA:RE16764; CG12881; CG31057; CG31058; CG34294; CG45110; CG5606; d-tau; DmelCG45110; dtau; dTau; Tau

Reactivity

Mouse

Pricing

diamine oxidase, DAO, ELISA Kit (Cat# AAA11096)

Full Name

Mouse diamine oxidase, DAO ELISA Kit

Reactivity

Mouse

Pricing

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WB (Western Blot) (Western BlotSample: Recombinant GAL9, Human;Antibody: Rabbit Anti-Human GAL9 Ab)

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Full Name

Active Galectin 9 (GAL9)

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>90%

Pricing

8-hydroxy-2-deoxyguanosine (8-OHdG), ELISA Kit (Cat# AAA13164)

Full Name

Rat 8-hydroxy-2-deoxyguanosine (8-OHdG) ELISA Kit

Reactivity

Rat

Pricing

Bovine Serum Albumin (BSA), ELISA Kit (Cat# AAA12913)

Full Name

Human Bovine Serum Albumin (BSA) ELISA Kit

Reactivity

Human

Pricing

complement factor properdin, ELISA Kit (Cat# AAA18290)

Full Name

Human Properdin, CFP ELISA Kit

Gene Names

CFP; BFD; PFC; PFD; PROPERDIN

Reactivity

Human

Pricing

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ASGR1, Polyclonal Antibody (Cat# AAA23426)

Full Name

ASGR1 Antibody - middle region

Gene Names

ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Yeast

Applications

WB

Purity

Affinity Purified

Pricing

Urea, Assay Kit (Cat# AAA11413)

Full Name

Urea Assay Kit

Pricing

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WB (Western Blot) (Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.)

HAO1/GOX, Monoclonal Antibody (Cat# AAA23994)

Full Name

HAO1/GOX (4B4) Mouse mAb

Gene Names

HAO1; GOX; GOX1; HAOX1

Reactivity

Mouse, Rat

Applications

WB, IHC-P, ICC, IF

Purity

Affinity purified

Pricing

Steroid 5 Alpha Reductase 2 (SRD5a2), ELISA Kit (Cat# AAA20662)

Full Name

Rat Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit

Reactivity

Rat

Pricing

Transcription Factor / Activator Protein 1 (AP-1), ELISA Kit (Cat# AAA22479)

Full Name

Human Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

Gene Names

JUNB; AP-1

Reactivity

Human

Pricing

Inhibin B, ELISA Kit (Cat# AAA17365)

Full Name

Mouse Inhibin B ELISA Kit

Reactivity

Mouse

Pricing

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Cyclin D1, Monoclonal Antibody (Cat# AAA28377)

Full Name

[KO Validated] Cyclin D1 Rabbit mAb

Gene Names

CCND1; BCL1; PRAD1; U21B31; D11S287E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using INTS6 antibody. Blue: DAPI for nuclear staining.)

INTS6, Polyclonal Antibody (Cat# AAA28131)

Full Name

INTS6 Polyclonal Antibody

Gene Names

INTS6; HDB; INT6; DBI-1; DDX26; DICE1; DDX26A; Notchl2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

Fibroblast Growth Factor 23 (FGF23), ELISA Kit (Cat# AAA13035)

Full Name

Porcine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

Gene Names

FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC

Reactivity

Porcine

Pricing

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WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)

DDX56, Monoclonal Antibody (Cat# AAA25077)

Full Name

DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (FITC)

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas)

SQSTM1, Polyclonal Antibody (Cat# AAA23550)

Full Name

SQSTM1 antibody - middle region

Gene Names

SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3

Reactivity

Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-PSAP antibody (AAA19741).Overlay histogram showing MCF-7 cells stained with AAA19741 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCF-7 Antibody (AAA19741, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of PSAP using anti-PSAP antibody (AAA19741).PSAP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSAP Antibody (AAA19741) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSAP Antibody (AAA19741) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAP antigen affinity purified polyclonal antibody (#AAA19741) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSAP at approximately 70 kDa. The expected band size for PSAP is at 58 kDa.)