Mouse ADAM metallopeptidase domain 8 ELISA Kit | ADAM8 elisa kit

Mouse A Disintegrin And Metalloprotease 8, ADAM8 ELISA Kit

Cat. Num. AAA80088

• Gene Names: Adam8; MS2; CD156; CD156a; E430039A18Rik
• Reactivity: Mouse
• Synonyms: ADAM metallopeptidase domain 8; N/A; Mouse A Disintegrin And Metalloprotease 8, ADAM8 ELISA Kit; Mouse A Disintegrin And Metalloprotease 8; ADAM8 ELISA Kit; CD156; MGC134985; MS2; a disintegrin and metalloproteinase domain 8; cell surface antigen MS2; disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen; ADAM8 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ADAM8. No significant cross-reactivity or interference between Mouse ADAM8 and analogues was observed.
• Sequence Length: 826
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for ADAM8 elisa kit

Introduction: ADAM metallopeptidase domain 8, also known as ADAM8, is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for ADAM8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6680642
• NCBI GeneID: 11501
• NCBI Accession #: NP_031429.1
• NCBI GenBank Nucleotide #: NM_007403.2
• UniProt Accession #: Q05910
• Molecular Weight: 90,046 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 8 isoform 1
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 8
• NCBI Official Symbol: Adam8
• NCBI Official Synonym Symbols: MS2; CD156; CD156a; E430039A18Rik
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8; ADAM 8; cell surface antigen MS2; macrophage cysteine-rich glycoprotein; a disintegrin and metalloprotease domain 8; a disintegrin and metalloprotease domain (ADAM) 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• UniProt Gene Name: Adam8
• UniProt Synonym Gene Names: Ms2; ADAM 8
• UniProt Entry Name: ADAM8_MOUSE