SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TBK1 recombinant protein
TBK1, Active
Gene Names
TBK1; NAK; T2K; FTDALS4
Applications
Western Blot
Synonyms
TBK1; N/A; TBK1, Active; NAK; T2K; FLJ11330; TBK1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for TBK1 recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for TBK1 recombinant protein
Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC034950.
TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases (1). Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon (2).
TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases (1). Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon (2).
Product Categories/Family for TBK1 recombinant protein
References
1. Tojima, Y. et al: NAK is an I-kappa-B kinase-activating kinase. Nature 404: 778-782, 2000.
2. Sharma, S. et al: Triggering the interferon antiviral response through an IKK-related pathway. Science 300: 1148-1151, 2003.
2. Sharma, S. et al: Triggering the interferon antiviral response through an IKK-related pathway. Science 300: 1148-1151, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
83,642 Da
NCBI Official Full Name
Homo sapiens TANK-binding kinase 1, mRNA
NCBI Official Synonym Full Names
TANK binding kinase 1
NCBI Official Symbol
TBK1
NCBI Official Synonym Symbols
NAK; T2K; FTDALS4
NCBI Protein Information
serine/threonine-protein kinase TBK1
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Product Notes
The TBK1 (Catalog #AAA73980) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TBK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the TBK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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