Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
185 results for "macrophages" - showing 1-50
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)
CD163, Monoclonal Antibody (Cat# AAA12154)
Full Name
MOUSE ANTI RAT CD163
Gene Names
CD163; M130; MM130
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(AAA30923 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Galectin 3, Polyclonal Antibody (Cat# AAA30923)
Full Name
Galectin 3 Antibody
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IL1RA, Polyclonal Antibody (Cat# AAA14336)
Full Name
IL1RA antibody
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Not determined
Applications
Western Blot
Pricing
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD200R: Low Endotoxin)
CD200R, Monoclonal Antibody (Cat# AAA12039)
Full Name
RAT ANTI MOUSE CD200R:RPE
Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26762)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (HRP)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)
CD68, Monoclonal Antibody (Cat# AAA12150)
Full Name
MOUSE ANTI RAT CD68:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
CD204, Monoclonal Antibody (Cat# AAA12080)
Full Name
RAT ANTI MOUSE CD204
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Application Data
(Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
CD172a, Monoclonal Antibody (Cat# AAA12056)
Full Name
MOUSE ANTI RAT CD172a:RPE
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
CD204, Monoclonal Antibody (Cat# AAA12076)
Full Name
RAT ANTI MOUSE CD204:FITC
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12117)
Full Name
RAT ANTI MOUSE CD206:Biotin
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11853)
Full Name
MOUSE ANTI RAT CD11b:Biotin
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry
Pricing
Application Data
(Published Customer Image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.Image caption:S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-mum-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A-C) S.Typhimurium (O-antigen, arrows) are red, macrophages (F4-80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-mum Z-stack. Scale bar is 20 mum. (B and C) Sections from (A) that are 4 mum apart. The video from which (A-C) were derived (Video S2) is available online. (D-G) T cells within multinucleate macrophages.Macrophages (F4-80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 mum. (H) An image from a different mouse stained and labeled as described for (D-G). Scale bar is 8 mum. A video showing a T cell inside of a macrophage is available online (Video S3).From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3(12): e193.)
Gr-1, Monoclonal Antibody (Cat# AAA12257)
Full Name
Rat Anti Mouse Gr-1: FITC
Reactivity
Mouse
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26737)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (Biotin)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(Frozen mouse spleen stained with Rat anti Mouse CD169)
CD169, Monoclonal Antibody (Cat# AAA12204)
Full Name
RAT ANTI MOUSE CD169
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12122)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13)
CD13, Monoclonal Antibody (Cat# AAA12144)
Full Name
RAT ANTI MOUSE CD13
Gene Names
Anpep; Apn; AP-M; AP-N; Cd13; P150
Applications
Immunohistochemistry, Flow Cytometry
Purity
Affinity chromatography on Protein G from tissue culture supernatant
Pricing
Application Data
(Frozen mouse spleen stained with Rat anti Mouse CD169)
CD169, Monoclonal Antibody (Cat# AAA12206)
Full Name
RAT ANTI MOUSE CD169:FITC
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12125)
Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12057)
Full Name
RAT ANTI MOUSE CD11b:RPE
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)
CD68, Monoclonal Antibody (Cat# AAA12149)
Full Name
MOUSE ANTI RAT CD68
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12120)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12227)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12165)
Full Name
RAT ANTI MOUSE F4/80:Low Endotoxin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)
CD68, Monoclonal Antibody (Cat# AAA12245)
Full Name
RAT ANTI MOUSE CD68:Endotoxin Low
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Application Data
(Frozen mouse spleen stained with Rat anti Mouse CD169)
CD169, Monoclonal Antibody (Cat# AAA12223)
Full Name
RAT ANTI MOUSE CD169:Low Endotoxin
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA11889)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rac1/cdc42, Polyclonal Antibody (Cat# AAA31342)
Full Name
Rac1/cdc42 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD163, Monoclonal Antibody (Cat# AAA12256)
Full Name
Mouse Anti Human CD163: RPE
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12182)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12173)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12146)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12170)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12184)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12045)
Full Name
MOUSE ANTI RAT CD11b:RPE
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11969)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:FITC)
CD36, Monoclonal Antibody (Cat# AAA12213)
Full Name
RAT ANTI MOUSE CD36
Gene Names
Cd36; FAT; GPIV; Scarb3
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistochemistry)
((4ug/ml) staining of paraffin embedded Human Lymph Node. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
ZCCHC11/PAPD3, Polyclonal Antibody (Cat# AAA13653)
Full Name
Goat anti-ZCCHC11/PAPD3 Antibody
Gene Names
ZCCHC11; TUT4; PAPD3
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
Peptide ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12171)
Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:FITC)
CD36, Monoclonal Antibody (Cat# AAA12214)
Full Name
RAT ANTI MOUSE CD36:Low Endotoxin
Gene Names
Cd36; FAT; GPIV; Scarb3
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12219)
Full Name
RAT ANTI MOUSE CD11b:Low Endotoxin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)
CD68, Monoclonal Antibody (Cat# AAA12147)
Full Name
MOUSE ANTI RAT CD68:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12168)
Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12166)
Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12123)
Full Name
RAT ANTI MOUSE CD206
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Frozen mouse spleen stained with Rat anti Mouse CD169)
CD169, Monoclonal Antibody (Cat# AAA12205)
Full Name
RAT ANTI MOUSE CD169:FITC
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12162)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12217)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12161)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12172)
Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13)
CD13, Monoclonal Antibody (Cat# AAA12145)
Full Name
RAT ANTI MOUSE CD13
Gene Names
Anpep; Apn; AP-M; AP-N; Cd13; P150
Applications
Immunohistochemistry, Flow Cytometry
Pricing