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16 results for "TGF beta Signaling" - showing 1-16
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Smad2, Polyclonal Antibody (Cat# AAA31390)
Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Smad2, Polyclonal Antibody (Cat# AAA31433)
Full Name
Phospho-Smad2 (Ser465+Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad1/9, Polyclonal Antibody (Cat# AAA31453)
Full Name
Phospho-Smad1/9 (Ser463/Ser465) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SMAD1 mAb. [Lot No. 913C1b-1])
SMAD1, Monoclonal Antibody (Cat# AAA10611)
Full Name
Mouse monoclonal antibody Anti-Human SMAD1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry
Pricing
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HeLa cells, using TGF beta Receptor I (Phospho-Ser165) antibody.)
TGF beta Receptor I, Antibody (Cat# AAA18002)
Full Name
TGF beta Receptor I (Phospho-Ser165) Antibody
Gene Names
TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; TBRI; ALK-5; LDS1A; LDS2A; TBR-i; TGFR-1; ACVRLK4; tbetaR-I
Reactivity
Human, Mouse, Rat
Applications
IF, EIA, FC/FACS
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(WB Suggested Anti-TGFB1 Antibody Titration: 0.2-1 ug/ml or 1:5000 to 1:1000 dilution.SP2/0 cell lysate)
TGFB1, Polyclonal Antibody (Cat# AAA23459)
Full Name
TGFB1 antibody - middle region
Gene Names
Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep (Tested Reactivity: Human, Mouse)
Applications
IHC, WB, IF
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
SMAD1, Monoclonal Antibody (Cat# AAA26193)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (Biotin)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
SMAD1, Monoclonal Antibody (Cat# AAA25927)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (AP)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 (Cat # L008V1).)
SMAD1, Monoclonal Antibody (Cat# AAA26459)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (HRP)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
Application Data
(AAA31434 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31434 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Smad3, Polyclonal Antibody (Cat# AAA31434)
Full Name
Phospho-Smad3 (Ser423+Ser425) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Sample: Recombinant BMP7, Mouse;Antibody: Rabbit Anti-Mouse BMP7 Ab)
Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA21057)
Full Name
Active Bone Morphogenetic Protein 7 (BMP7)
Gene Names
Bmp7; OP1
Reactivity
Mus musculus (Mouse)
Applications
Cell Culture, Activity Assay
Purity
>98%
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
SMAD1, Monoclonal Antibody (Cat# AAA26060)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (APC)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
IF, IHC, WB
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 (Cat # L008V1).)
SMAD1, Monoclonal Antibody (Cat# AAA26326)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (FITC)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
IF, IHC, WB
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 (Cat # L008V1).)
SMAD1, Monoclonal Antibody (Cat# AAA26592)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (PE)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
IF, IHC, WB
Purity
Purified
Pricing