Search results for "SARS related"

6 results for "SARS related" - showing 1-6

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SDS-PAGE (SDS-PAGE of Nucleocapsid Protein-His in non related (NR) and reduced (R) condition.)

COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA27966)

Full Name

Nucleocapsid Protein-His (HEK293)

Purity

>90%

Pricing

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IF (Immunofluorescence) (Figure 11 Immunofluorescence Validation of ACE2 In Caco2 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10945 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)

IF (Immunofluorescence) (Figure 9 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with AAA10945 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 8 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot) (Figure 4 Western Blot Validation in Rat Thymus Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, AAA10945 (2 ug/mL) and beta-actin (1 ug)

IF (Immunofluorescence) (Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with AAA10945 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)

ACE2, Polyclonal Antibody (Cat# AAA10945)

Full Name

ACE2 Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ACE2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immuofluorescence) (Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10928 at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)

IF (Immunofluorescence) (Figure 8 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with AAA10928 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with AAA10928 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunfluorescence) (Figure 6 Immunofluorescence Validation of ACE2 in Human Test is Tissue Immunofluorescent analysis of 4% paraformalde hyde-fixed human test is tissue labeling ACE-2 with AAA10928 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (AAA10928) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Figure 4 Western Blot Validation in Rat Skin Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug))

ACE2, Polyclonal Antibody (Cat# AAA10928)

Full Name

ACE2 Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ACE2 Antibody is affinity chromatography purified via peptide column.

Pricing

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Application Data (Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)

Application Data (Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/HEK293 stable cells and parental HEK293 cells, and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets, and the plasmid DNA of target gene construct was used as positive control.)

Application Data (Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.)

Application Data (Fig-2: Detection of ACE2 expression in ACE2/HEK293 cells by flow cytometry. ACE2/HEK293 cells were probed with biotinylated SARS-Cov-2 Spike RBD protein at 1 x 10^5 cells per 1 ug protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.)

Application Data (Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)

ACE2, Cell Line (Cat# AAA14888)

Full Name

ACE2/HEK293 Stable Cell Line

Gene Names

ACE2; ACEH

Applications

Functional Assay

Pricing

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Biological Activity (Binding of anti-2019nCoV spike protein antibody (AAA14259) to 2019nCoV RBD determined by ELISA.)

FCM (Flow Cytometry) (FACS assay showed 2019-nCoV Spike protein RBD and 2019-nCoV Spike protein S1 can bind to ACE2 overexpressing cells (Panel A and B respectively). ACE2 overexpressing cells were stained with 2019-nCoV Spike protein RBD or S1, followed by anti-Spike protein antibody and fluorescence-conjugated secondary antibody.)

COVID 19 Spike Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA14259)

Full Name

Anti-2019nCoV Spike Protein, Recombinant Human Antibody

Reactivity

SARS-CoV spike protein

Applications

Flow Cytometry

Pricing

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IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (AAA28762), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-ACE2 C-term Pab (AAA28762)in K562 cell line lysates (35ug/lane). ACE2(arrow) was detected using the purified Pab.)

WB (Western Blot) (The anti-ACE2 C-term Pab (AAA28762) is used in Western blot to detect ACE2 in 293 cell lysate.)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

Full Name

ACE2 (SARS Receptor) Antibody (C-term)

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

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6 results for "SARS related" - showing 1-6

COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA27966)

ACE2, Polyclonal Antibody (Cat# AAA10945)

ACE2, Polyclonal Antibody (Cat# AAA10928)

ACE2, Cell Line (Cat# AAA14888)

COVID 19 Spike Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA14259)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

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6 results for "SARS related" - showing 1-6

COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA27966)

ACE2, Polyclonal Antibody (Cat# AAA10945)

ACE2, Polyclonal Antibody (Cat# AAA10928)

ACE2, Cell Line (Cat# AAA14888)

COVID 19 Spike Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA14259)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

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