Search results for "Reagents Buffer"

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IF (Immunofluorescence) (Immunofluorescentstaining of PFA-fixedHeLa cells with Pan-Nuclear Antigen Monoclonal Antibody (AAA13844) followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).)

Nuclear Antigen, Monoclonal Antibody (Cat# AAA13844)

Full Name

Nuclear Antigen (Pan-Nuclear Marker) Mouse Monoclonal Antibody

Reactivity

Human, Mouse, Rat.
Does not react with Pig

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Pricing

Carcinoembryonic Antigen (CEA) Antigen Grade, Native Protein (Cat# AAA13426)

Full Name

CEA Antigen Grade

Gene Names

CEACAM6; NCA; CEAL; CD66c; CEACAM6

Applications

ELISA

Purity

Immunoaffinity purification. SDS-PAGE denaturing: multiple protein bands 150-210kDa

Pricing

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Application Data

Tryptase, Monoclonal Antibody (Cat# AAA13565)

Full Name

Tryptase

Gene Names

MCT7; PMCT7; TPSB1

Reactivity

Human

Applications

Immunohistochemistry

Pricing

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CA15-3, Monoclonal Antibody (Cat# AAA13555)

Full Name

CA15-3

Reactivity

Paraffin, frozen

Applications

Immunohistochemistry, Immunohistochemistry

Pricing

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QC (Quality Control)

Treponema pallidum, Polyclonal Antibody (Cat# AAA13577)

Full Name

Treponema pallidum

Reactivity

Eubacteria

Applications

Immunohistochemistry

Pricing

Beta-2 Glycoprotein I, Apolipoprotein H (Apo H), Native Protein (Cat# AAA13420)

Full Name

Human Beta-2 gp I, Apo H

Gene Names

APOH; BG; B2G1; B2GP1

Applications

ELISA

Purity

>95% pure (SDS-PAGE). Column chromatography

Pricing

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Macrophage HAM-56, Monoclonal Antibody (Cat# AAA13562)

Full Name

Macrophage HAM-56

Reactivity

Human, Monkey

Applications

Immunohistochemistry

Pricing

Carbonic Anhydrase IX, Monoclonal Antibody (Cat# AAA14680)

Full Name

Carbonic Anhydrase IX (CA9) (PE)

Gene Names

CA9; MN; CAIX

Applications

Flow Cytometry

Purity

Affinity Purified
Purified by Protein A affinity chromatography from hybridoma culture supernant.

Pricing

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Staining Procedure (1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)