Search results for "Primary antibodies Microbiology"

6 results for "Primary antibodies Microbiology" - showing 1-6

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WB (Western Blot) (PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)

WB (Western Blot) (Western blot analysis of lysates from human ovary and mouse testis tissue lysates (from left to right), using PLD6 Antibody (Center)(Cat# AAA28684). AAA28684 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

PLD6, Polyclonal Antibody (Cat# AAA28684)

Full Name

PLD6 Antibody (Center)

Gene Names

PLD6; ZUC

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (N-term). AAA28776 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term). AAA28776 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

Full Name

GAPDH Antibody (N-term)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Figure 12 Overexpression Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, AAA10931 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.)

IHC (Immunohistochemistry) (Figure 11 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Brain Spike protein was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the brain of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistochemistry) (Figure 10 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Lung Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the lung of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistchemistry) (Figure 9 IHC Validation of SARS-CoV2 Spike in the Nasopharyngeal Swab Sample of the COVID-19 Patient Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the nasopharyngeal swab sample of the COVID-19 patient and no spike signal was observed in the sample of the COVID-19 negative patient. COVID-19 cases were confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IF (Immunofluorescence) (Figure 8 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Skin (Magro et al., 2020) C4d is highlighted green while COVID-19 spike protein detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) shows a red staining pattern; a yellow signal is discernible indicative of co-localization of C4d and viral protein within the microvasculature.)

IF (Immunofluorescence) (Figure 7 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Lung (Magro et al., 2020) SARS-CoV2 spike protein (red, panel C) detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) colocalized with C4d (green in panel d, merged in yellow). Spike protein (red, panel g) was also colocalized with C5b-9 (green in panel f&h, merged in yellow))

IHC (Immunohistchemistry) (Figure 6 IHC/IF Validation in COVID-19 Patient Sample (Nuovo et al., 2020) Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations. B. An intense signal for covid-19 spike protein tested by SARS-CoV-2 spike antibodies (AAA10931) was observed in the glandular cells. F-H. Co-expression of spike detected by spike antibodies (AAA10931, 0.2 ug/mL) and envelope proteins detected by envelope antibodies (, 2 ug/mL) of SARSCoV- 2 (F) documented localization of each protein to glandular cells (G, yellow). No signal was seen in oral swabs of positive cases (H). Both the spike and envelope protein detected by anti-spike antibodies (AAA10931) and anti-envelope antibodies (,) produced a signal in the nasopharyngeal swabs of the three cases and no signal was evident in the nasopharyngeal swabs of the seven controls.)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal wasobserved in macrophages of COVID-19 patient lung.)

IHC (Immunohistochemistry) (Figure 4 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

IF (Immunofluorescence) (Figure 3 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021) Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k-s) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k-m); pan-macrophage marker CD68 (magenta) (n-p); HLA-DR (magenta) and pDC marker CD123 (yellow) (q–s) and DAPI (blue).)

IF (Immunofluorescence) (Figure 2 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Nose and Tonsil (Singh et al., Nature Microbiology, 2021) Multi-label confocal immunofluorescence microscopy of nasal epithelium (20X-b, 63xh) and tonsil (20X-c,63X-i) from rhesus macaques infected with SARS-CoV-2 with SARS-CoV-2 spike-specific antibodies, AAA10931. (turquoise), DAPI (blue). Rabbit IgG isotype control antibody was used to stain the tissues to rule out any nonspecific staining (e, f).)

IF (Immunofluorescence) (Firure 1. Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

Full Name

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody

Reactivity

Virus

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.

Pricing

WB (Western Blot) (WB of AAA14403 with 1:500 antibody dilution in DiluObuffer. Apparent MW: 48)

FtsZ, Polyclonal Antibody (Cat# AAA14403)

Full Name

FtsZ Antibody

Applications

Immunoprecipitation, Western Blot

Pricing

Staphylococcal Enterotoxin B, Monoclonal Antibody (Cat# AAA14625)

Full Name

mAb anti-Staphylococcal Enterotoxin B [SEB]

Reactivity

Staphylococcal enterotoxin B

Applications

ELISA

Purity

Protein G affinity purified

Pricing

WB (Western Blot) (All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

ERVK-7, Polyclonal Antibody (Cat# AAA28797)

Full Name

ERVK-7 Antibody (N-Term)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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6 results for "Primary antibodies Microbiology" - showing 1-6

PLD6, Polyclonal Antibody (Cat# AAA28684)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

FtsZ, Polyclonal Antibody (Cat# AAA14403)

Staphylococcal Enterotoxin B, Monoclonal Antibody (Cat# AAA14625)

ERVK-7, Polyclonal Antibody (Cat# AAA28797)

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6 results for "Primary antibodies Microbiology" - showing 1-6

PLD6, Polyclonal Antibody (Cat# AAA28684)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

FtsZ, Polyclonal Antibody (Cat# AAA14403)

Staphylococcal Enterotoxin B, Monoclonal Antibody (Cat# AAA14625)

ERVK-7, Polyclonal Antibody (Cat# AAA28797)

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