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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
135 results for "Mouse Anti Rat IgG2b" - showing 1-50
IF (Immunofluorescence)
(BALB/c mouse splenocytes were stained with Rat Anti-Mouse Lamda-PE and Rat Anti-Mouse CD119-FITC.)
Rat Anti-Mouse Lambda (lambda light chain specific), Monoclonal Secondary Antibody (Cat# AAA14893)
Full Name
Rat Anti-Mouse Lambda-UNLB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.)
CD4, Monoclonal Antibody (Cat# AAA12278)
Full Name
RAT ANTI DOG CD4: APC
Reactivity
Dog
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26762)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (HRP)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
S100A8, Monoclonal Antibody (Cat# AAA14670)
Full Name
S100A8 (MRP-8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
CD204, Monoclonal Antibody (Cat# AAA12080)
Full Name
RAT ANTI MOUSE CD204
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
CD204, Monoclonal Antibody (Cat# AAA12076)
Full Name
RAT ANTI MOUSE CD204:FITC
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))
CD45, Monoclonal Antibody (Cat# AAA12021)
Full Name
RAT ANTI MOUSE CD45:RPE
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
Flow Cytometry
Pricing
Application Data
(Published Customer Image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.Image caption:S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-mum-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A-C) S.Typhimurium (O-antigen, arrows) are red, macrophages (F4-80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-mum Z-stack. Scale bar is 20 mum. (B and C) Sections from (A) that are 4 mum apart. The video from which (A-C) were derived (Video S2) is available online. (D-G) T cells within multinucleate macrophages.Macrophages (F4-80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 mum. (H) An image from a different mouse stained and labeled as described for (D-G). Scale bar is 8 mum. A video showing a T cell inside of a macrophage is available online (Video S3).From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3(12): e193.)
Gr-1, Monoclonal Antibody (Cat# AAA12257)
Full Name
Rat Anti Mouse Gr-1: FITC
Reactivity
Mouse
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26737)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (Biotin)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12057)
Full Name
RAT ANTI MOUSE CD11b:RPE
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12227)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))
CD45, Monoclonal Antibody (Cat# AAA11856)
Full Name
RAT ANTI MOUSE CD45:FITC
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12165)
Full Name
RAT ANTI MOUSE F4/80:Low Endotoxin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P Samples:Human Prostate Gland Tissue))
Dickkopf Related Protein 1, Monoclonal Antibody (Cat# AAA20822)
Full Name
Dickkopf Related Protein 1 (DKK1)
Gene Names
DKK1; SK; DKK-1
Reactivity
Human, Mouse
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Purification: Protein A + Protein G affinity chromatography
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA11889)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12182)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.)
PBK/TOPK, Monoclonal Antibody (Cat# AAA14113)
Full Name
Anti-PBK/TOPK Mouse mAb
Gene Names
PBK; SPK; CT84; TOPK; HEL164; Nori-3
Reactivity
Human, Rat
Applications
Western Blot
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12173)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
YWHAZ, Monoclonal Antibody (Cat# AAA27050)
Full Name
YWHAZ Monoclonal Antibody
Gene Names
YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein A purified
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12170)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12184)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12171)
Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12219)
Full Name
RAT ANTI MOUSE CD11b:Low Endotoxin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12168)
Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12166)
Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12162)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12161)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12172)
Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12217)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow cytometry of Jurkat cells, using anti-BSG antibody, (Red), compared to a nonspecific negative control antibody, (Blue).)
Basigin / Emmprin / CD147, Monoclonal Antibody (Cat# AAA12375)
Full Name
Anti-Basigin / Emmprin / CD147 Antibody (clone 10E10) IHC-plus
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Dog, Rat, Human, Monkey
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A/G purified
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26723)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (AP)
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4)
CD4, Monoclonal Antibody (Cat# AAA11939)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(MARCH7 monoclonal antibody. Western Blot analysis of 'MARCH7 expression in PC-12.)
MARCH7, Monoclonal Antibody (Cat# AAA24075)
Full Name
MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
Application Data
(Overlay histogram showing SY5Y cells stained with AAA27008 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (10ug/1x10^6cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
GFAP, Monoclonal Antibody (Cat# AAA27008)
Full Name
GFAP Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))
CD45, Monoclonal Antibody (Cat# AAA11915)
Full Name
RAT ANTI MOUSE CD45
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12230)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12183)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12185)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
WB (Western Blot)
GAA, Monoclonal Antibody (Cat# AAA19211)
Full Name
Anti-GAA Antibody (Monoclonal, 2G7)
Gene Names
GAA; LYAG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26786)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (MaxLight 405)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12187)
Full Name
RAT ANTI MOUSE CD11b:Biotin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12169)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of >10,000 events was performed.)
HSPA8, Monoclonal Antibody (Cat# AAA27045)
Full Name
HSPA8 Monoclonal Antibody
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12163)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
Beta-Tubulin, Monoclonal Antibody (Cat# AAA27713)
Full Name
Beta-Tubulin Loading Control Antibody, Mouse MAb
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12174)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))
CD45, Monoclonal Antibody (Cat# AAA12063)
Full Name
RAT ANTI MOUSE CD45
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12164)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))
CD45, Monoclonal Antibody (Cat# AAA11914)
Full Name
RAT ANTI MOUSE CD45
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA28066 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ACTB, Monoclonal Antibody (Cat# AAA28066)
Full Name
ACTB Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing