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Gene Name
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Application
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11 results for "M Tuberculosis" - showing 1-11
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
ESAT-6, Polyclonal Antibody (Cat# AAA20027)
Full Name
Anti-ESAT-6 (M. tuberculosis) Purified
Gene Names
esxA; esat-6
Reactivity
Mycobacterium tuberculosis
Applications
Western Blot
Purity
Purified by ligand affinity chromatography
Pricing
CFP-10, Polyclonal Antibody (Cat# AAA20026)
Full Name
Anti-CFP-10 (M. tuberculosis) Purified
Gene Names
esxB; cfp10; lhp
Reactivity
Mycobacterium tuberculosis
Applications
Western Blot
Purity
Purified by ligand affinity chromatography
Pricing